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P5899

Sigma-Aldrich

Poly-ʟ-Lysine Hydrobromide

synthetic, mol wt ≥300,000, powder, γ-irradiated, suitable for cell culture, BioXtra

Sinonimo/i:

PDL HBr

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About This Item

Numero CAS:
25988-63-0
Numero MDL:
MFCD00237230
Codice UNSPSC:
12352202
eCl@ss:
32160406
ID PubChem:
24898718
NACRES:
NA.75

Nome del prodotto

Poli-L-lisina, mol wt ≥300,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

Origine biologica

synthetic (organic)

Livello qualitativo

200

Sterilità

γ-irradiated

Nome Commerciale

BioXtra

Stato

lyophilized powder

PM

≥300,000

Confezionamento

pkg of 5 mg

tecniche

cell culture | mammalian: suitable

Copertura della superficie

4 μg/cm2

Impurezze

<10% water (by Karl Fischer)

Solubilità

H2O: soluble 50 mg/mL, clear to very slightly hazy, colorless to faintly yellow

Condizioni di spedizione

ambient

Temperatura di conservazione

−20°C

Stringa SMILE

Cl.NCCCCC(N)C(O)=O

InChI

1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1
WBSCNDJQPKSPII-KKUMJFAQSA-N

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Applicazioni

Poly-L-lysine hydrobromide has been used to coat:
  • 2-well plates before seeding muscle cells in a research study on rainbow trout myotube formation
  • flat bottom 96-well plates for in vitro neuronal stem cell differentiation
  • T75 flasks for primary astrocytic cultures

Azioni biochim/fisiol

Poly-L-lysine serves as a coating substance to ensure better attachment of cells to culture surfaces. Research shows that plates coated with poly-L-lysine (PLL) demonstrated an accelerated cell growth rate. Culturing mesenchymal stem cells (MSCs) on PLL-coated substrates has been found to safeguard the stemness properties of these cells and inhibit senescence. PLL-coated plates have demonstrated enhanced MSC growth and up-regulated the expression of genes associated with cell adhesion, differentiation, proliferation, and signaling. Moreover, porous PLL-coated surfaces have shown improvements in cell proliferation and the osteogenic differentiation of MSCs.

Componenti

La poli-L-lisina è un polimero aminoacidico con carica positiva con circa un HBr per residuo di lisina. Il bromidrato consente alla poli-L-lisina di raggiungere la forma cristallina solubile in acqua. Una piccola quantità del prodotto può essere rinvenuta nella struttura beta perché il HBr interferisce con il legame idrogeno tra i gruppi amminici e i gruppi carbossilici o le frazioni che contengono N o O.

Avvertenza

Le soluzioni sterili sono stabili per un massimo di due anni se conservate a 2-8 °C. Il prodotto deve essere conservato essiccato a -20 °C.

Risultati analitici

Molecular weight based on viscosity. Also assayed by MALLS.

Altre note

For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
0000315801
0000315801
SLCL5836
SLCF0030
SLBT1729

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Journal of Separation Science, 5(7) (2021)
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Purpose. The purpose of this work was to evaluate the retinal integration and differentiation of neurospheres formed by stem cells and mouse neural progenitor cells injected intravitreally in mice eyes with retinal injury. Methods. Eight male C57BL mice, 8 weeks
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Between cell divisions the chromatin fiber of each chromosome is restricted to a subvolume of the interphase cell nucleus called chromosome territory. The internal organization of these chromosome territories is still largely unknown. We compared the large-scale chromatin structure of
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Visualizza tutti i documenti correlati

Articoli

Poly-lysine Types for Biomedical Applications

Poly-Lysine enhances cell binding with positively-charged surface ions, optimizing electrostatic interaction on culture surfaces for increased cell attachment.

Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Cancer Stem Cells: Targets for Cancer Therapy

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

Protocolli

Poly-L-Lysine Cell Attachment Protocol

Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.

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