P5511
Protein Kinase A from bovine heart
≥0.8 units/μg protein, lyophilized powder
Sinonimo/i:
Protein Kinase, 3′,5′-cyclic-AMP-dependent from bovine heart
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Scegli un formato
1 MG
CHF 48.10
5 MG
CHF 136.00
10 MG
CHF 253.00
Scegli un formato
Cambia visualizzazione
1 MG
CHF 48.10
5 MG
CHF 136.00
10 MG
CHF 253.00
About This Item
Numero CAS:
Numero MDL:
Codice UNSPSC:
12352204
eCl@ss:
32160410
NACRES:
NA.77
Prodotti consigliati
Stato
lyophilized powder
Attività specifica
≥0.8 units/μg protein
Composizione
Protein, ≥70%
Temperatura di conservazione
−20°C
Informazioni sul gene
cow ... PRKACA(282322) , PRKACB(282323)
Cerchi prodotti simili? Visita Guida al confronto tra prodotti
Descrizione generale
Protein Kinase A (PKA) is a serine/threonine kinase, which exists as a tetrameric holoenzyme.[1]
Applicazioni
Protein Kinase A from bovine heart has been used to determine its activity using a molecular beacon probe. It has also been used to study the interaction of staurosporine with the ATP-binding site of kinases.[2][3]
Protein Kinase A from bovine heart has been used to inhibit the phosphorylation of myosin.[4]
Azioni biochim/fisiol
Many 3′,5′-cyclic AMP dependent protein kinases have been reported. Structural studies (Traugh, J.A., et al., Meth. Enzymol., 38, 290 [1974]) show the presence of at least two subunits, the regulatory subunit and the catalytic subunit. When both units are linked together, the catalytic activity is inhibited. However, when the cyclic-AMP binds to the regulatory subunit, the catalytic subunit is released and can then catalyze the transfer of phosphate from ATP to various proteins.
Confezionamento
Package size based on protein content
Definizione di unità
One unit will transfer 1.0 picomole phosphate from ATP to hydrolyzed and partially dephosphorylated casein per minute at pH 6.5 at 30°C in the presence of cyclic AMP, determined by measuring the production of ADP.
Stato fisico
Crude lyophilized powder containing EDTA and potassium phosphate, pH 7.5.
Nota sulla preparazione
Fractionated essentially by procedure of Gilman, A., Proc. Natl. Acad. Sci. USA, 67, 305 (1970).
Risultati analitici
Protein determined by biuret.
Inibitore
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Scegli una delle versioni più recenti:
Certificati d'analisi (COA)
Lot/Batch Number
Non trovi la versione di tuo interesse?
Se hai bisogno di una versione specifica, puoi cercare il certificato tramite il numero di lotto.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
I clienti hanno visto anche
Alkylation of Staurosporine to Derive a Kinase Probe for Fluorescence Applications
Dr. Alexandar
Chemistry of Materials (2016)
Simple fluorescence-based detection of protein kinase A activity using a molecular beacon probe
Journal of Bioenergetics and Biomembranes (2017)
Cardiomyocyte Hypocontractility and Reduced Myofibril Density in End-Stage Pediatric Cardiomyopathy.
Ilse A E Bollen et al.
Frontiers in physiology, 8, 1103-1103 (2018-01-10)
Dilated cardiomyopathy amongst children (pediatric cardiomyopathy, pediatric CM) is associated with a high morbidity and mortality. Because little is known about the pathophysiology of pediatric CM, treatment is largely based on adult heart failure therapy. The reason for high morbidity
Signaling the signal: Protein Kinase a (PKA) inhibition by insulin-formed H2O2 and reactivation by thioredoxin
de Pina MZ, et al.
The Journal of Biological Chemistry (2008)
Nitroglycerine-and isoprenaline-induced vasodilatation: assessment from the actions of cyclic nucleotides
Itohy T, et al.
British Journal of Pharmacology, 84(2), 393-406 (1985)
Active Filters
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.
