P0393

Protein Kinase Inhibitor from porcine heart

1-3 inhibitor U/μg protein, lyophilized powder

• UNSPSC Code: 12352202
• MDL number: MFCD00132115

Proprietà

• biological source: Porcine heart
• form: lyophilized powder
• specific activity: 1-3 inhibitor U/μg protein
• composition: Protein, ~50% biuret
• storage temp.: 2-8°C

Descrizione

Biochem/physiol Actions

These proteins bind to the catalytic subunit of cyclic-AMP dependent protein kinase, displacing the regulatory subunit and inhibiting phosphorylating activity. The cyclic-AMP binding capacity of the regulatory subunit is enhanced. Many other proteins exhibit this enhancement ability, too (Brostrom, C.O. and Kon, C., Anal. Biochem., 58, 459 [1974]).

Packaging

Package size based on protein content.

Physical form

Supplied as lyophilized powder containing 30% glycerophosphate and 20% EDTA.

Preparation Note

Fractionated essentially by procedure of Walsh, D.A., et al., J. Biol. Chem., 246, 1977 (1971).

Other Notes

One unit will transfer 1.0 picomole (10^-12 mol) of phosphate from γ-³²P-ATP to hydrolyzed and partially dephosphorylated casein (Product No. C 4765) per min at pH 6.5 at 30 °C.

Informazioni sulla sicurezza

• Classe di stoccaggio: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)