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A8456

Sigma-Aldrich

4-(2-Aminoethyl)benzolsulfonylfluorid -hydrochlorid

≥97.0% (HPLC)

Synonym(e):

AEBSF

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25 MG
CHF 71.80
100 MG
CHF 197.00
500 MG
CHF 667.00

CHF 71.80

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25 MG
CHF 71.80
100 MG
CHF 197.00
500 MG
CHF 667.00

About This Item

Empirische Formel (Hill-System):
C8H10FNO2S · HCl
CAS-Nummer:
30827-99-7
Molekulargewicht:
239.69
Beilstein:
7604627
MDL-Nummer:
MFCD00132962
UNSPSC-Code:
12352202
PubChem Substanz-ID:
24278240
NACRES:
NA.77

CHF 71.80

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Biologische Quelle

synthetic (organic)

Qualitätsniveau

300

Assay

≥97.0% (HPLC)

Form

powder

mp (Schmelzpunkt)

183-191  °C

Löslichkeit

H2O: 50 mg/mL

Lagertemp.

−20°C

SMILES String

FS(C1=CC=C(CCN)C=C1)(=O)=O.[H]Cl

InChI

1S/C8H10FNO2S.ClH/c9-13(11,12)8-3-1-7(2-4-8)5-6-10;/h1-4H,5-6,10H2;1H

InChIKey

WRDABNWSWOHGMS-UHFFFAOYSA-N

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Allgemeine Beschreibung

Bei Kühllagerung bei einem pH-Wert von weniger als 7 bis zu 6 Monate stabil. Wenn ein pH-Wert von über 7 erforderlich ist, sollte der pH-Wert erst unmittelbar vor der Verwendung eingestellt werden.

Anwendung

4-(2-Aminoethyl)benzolsulfonylfluorid-hydrochlorid (AEBSF) wird wie folgt verwendet:
  • als Proteaseinhibitor in Plasmaproben zum Verhindern des Abbaus von acetyliertem Ghrelin (AG)
  • als Schlangengift-Serinproteinase(SVSP)-Inhibitor im SVSP-Hemmungs- oder AEBSF-Assay zum Prüfen der Beteiligung von SVSPs auf die Fibrinogengerinnungsaktivität
  • als Komponente von NP-40-Lysepuffer zum Resuspendieren von Zellrückständen zum Herstellen von Zelllysaten

Empfohlene Konzentration für den Einsatz: 0,1-1 mM.

Biochem./physiol. Wirkung

4-(2-Aminoethyl)benzolsulfonylfluoridhydrochlorid (AEBSF) ist ein irreversibler Serinproteaseinhibitor. Die Inhibitionskonstanten ähneln denen von Phenylmethansulfonylfluorid (PMSF) und Diisopropylfluorphosphat (DFP). AEBSF hemmt nachweislich Trypsin, Chymotrypsin, Plasmin, Kallikrein und Thrombin. Als Alternative zu PMSF und DFP bietet AEBSF eine geringere Toxizität, bessere Wasserlöslichkeit und bessere Stabilität in wässrigen Lösungen. AEBSF kann in Zellkulturen zu Konzentrationen von bis zu 0,25 mmol/l eingesetzt werden.
AEBSF kann Acetylcholinesterase (AChE) hemmen.

Piktogramme

Corrosion
GHS05

Signalwort

Danger

H-Sätze

H314

Gefahreneinstufungen

Eye Dam. 1 - Skin Corr. 1A

Lagerklassenschlüssel

8A - Combustible corrosive hazardous materials

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
0000409162
0000409162
0000389739
0000389740
0000359853

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Die Dokumentenbibliothek aufrufen

Ilario Mennella et al.
The Journal of nutrition, 145(9), 2169-2175 (2015-07-17)
Food palatability increases food intake and may lead to overeating. The mechanisms behind this observation are still largely unknown. The aims of this study were the following: 1) to elucidate the plasma responses of endocannabinoids, N-acylethanolamines, and gastrointestinal peptides to
Ignacio Pedrós et al.
Biochimica et biophysica acta, 1842(9), 1556-1566 (2014-06-03)
The present study had focused on the behavioral phenotype and gene expression profile of molecules related to insulin receptor signaling in the hippocampus of 3 and 6 month-old APPswe/PS1dE9 (APP/PS1) transgenic mouse model of Alzheimer's disease (AD). Elevated levels of
Xue Zhang et al.
Frontiers in plant science, 11, 277-277 (2020-03-29)
Post-translational covalent modifications of histones play important roles in modulating chromatin structure and are involved in the control of multiple developmental processes in plants. Here we provide insight into the contribution of the histone lysine methyltransferase SET DOMAIN GROUP 8
Niina Aaltonen et al.
Biological procedures online, 22, 6-6 (2020-03-20)
Serine hydrolases (SHs) are a functionally diverse family of enzymes playing pivotal roles in health and disease and have emerged as important therapeutic targets in many clinical conditions. Activity-based protein profiling (ABPP) using fluorophosphonate (FP) probes has been a powerful
Amy M Weeks et al.
Current protocols in chemical biology, 12(1), e79-e79 (2020-02-20)
Subtiligase is a powerful enzymatic tool for N-terminal modification of proteins and peptides. In a typical subtiligase-catalyzed N-terminal modification reaction, a peptide ester donor substrate is ligated onto the unblocked N terminus of a protein, resulting in the exchange of
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Artikel

ReadyShield Phosphatase and Protease Inhibitor Cocktail FAQ

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

Chymotrypsin

Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.

Questions

1–6 of 6 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. In what solvents is Product A8456, 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, soluble?

    1 answer

    1. It is highly soluble in water and in DMSO, at 50 mg/mL (approximately 200 mM), yielding a clear colorless solution. AEBSF may be used in cell culture medium at concentrations up to 0.25 mM.

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  4. What is the recommended working concentration for Product A8456, 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride?

    1 answer

    1. Our recommended working concentration range is 0.1 mM to 1 mM; the optimal concentration will depend on the application.

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  5. How stable is Product A8456, 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, in solution?

    1 answer

    1. Solutions in water are slightly acidic and retain inhibitory activity for up to six months when stored refrigerated. Solutions at pHs above 7 are less stable.  Stock solutions should be stored at a pH less than 7. If a final pH of greater than 7 is required, the pH adjustment should be done shortly before use.

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  6. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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