T9378

Trypsin inhibitor

crude powder

• Sinônimo(s): Trypsin Inhibitor from lima beans
• Número CAS: 9035-81-8
• Número CE: 232-906-9
• Número MDL: MFCD00082102
• Código UNSPSC: 12352202
• NACRES: NA.77

Propriedades

• Nome do produto: Trypsin inhibitor from Phaseolus limensis (lima bean), Type II-L, crude powder
• fonte biológica: Phaseolus limensis (lima bean)
• Nível de qualidade: 200
• tipo: Type II-L
• Formulário: crude powder
• peso molecular: 9 kDa
• solubilidade: 0.067 M sodium phosphate buffer, pH 7.6: 1 mg/mL
• temperatura de armazenamento: 2-8°C

Descrição

Aplicação

Trypsin inhibitor from Phaseolus limensis (lima bean) has been used:

• in the inhibition of trypsin in human plasma[1]
• in the termination of proteolytic digestion in rabbit skeletal muscles[2]
• in the inhibition of salivary glands enzyme extract from Lygus lineolaris[3]
• in the inhibition of proteolytic activity in Agave tequilana enzyme extract[4]

Trypsin inhibitor has been used as an affinity ligand for isolation of elastase-type[5] enzymes.

Ações bioquímicas/fisiológicas

Trypsin inhibitor from lima beans belongs to Bowman-Birk family of protease inhibitors.[6] It is 9 kDa in molecular weight and contains nine disulfide bridges and is highly thermostable. It has trypsin and chymotrypsin binding subdomains.[7]

propriedades físicas

Monomer has an apparent molecular weight of approx. 9,000 Da but undergoes a concentration and pH-dependent dimerization.

Definição da unidade

One trypsin unit will produce a ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.

Nota de preparo

Prepared by a modification of the method of Tauber, H., et al., J. Biol. Chem., 179, 1155 (1949) (modified).

Nota de análise

One mg will inhibit ≥0.8 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.

Outras notas

View more information on Trypsin Inhibitor.

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H317,H334
• Declarações de precaução: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Classificações de perigo: Resp. Sens. 1 - Skin Sens. 1
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)