T2327
Trypsin inhibitor
lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)
Sinônimo(s):
Kunitz Inhibitor
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About This Item
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product name
Trypsin Inhibitor from Glycine max (soybean), BioUltra, lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)
fonte biológica
Glycine max (soybean)
linha de produto
BioUltra
Ensaio
≥95% (Kunitz inhibitor, SDS-PAGE)
forma
lyophilized powder
temperatura de armazenamento
2-8°C
Categorias relacionadas
Descrição geral
Trypsin Inhibitor from Glycine max (soybean) also known as Kunitz trypsin inhibitor is a 21 kDa protein with a single trypsin binding reactive site.
Aplicação
Trypsin Inhibitor from Glycine max (soybean) has been used:
- as a standard protein to measure the amount of endogenous trypsin inhibitor present in midgut lysate (M1) of Riptortus pedestris
- as a standard to compare the trypsin inhibitory activity of the purified protein
- to monitor the trypsin inhibitory activity by fractionating in MonoS cation exchange chromatography
- as an trypsin inhibitor
Ações bioquímicas/fisiológicas
Soybean trypsin inhibitor inhibits trypsin and to a lesser extent chymotrypsin and plasmin. It forms a 1:1 stoichiometric complex with trypsin. Upon formation of this complex, trypsin may cleave a single arginine-isoleucine bond in the inhibitor. Dissociation of this complex may yield the modified form or the native inhibitor. At the optimal pH for trypsin binding (pH 8.0), the association constant is ≥ 10x108.
Trypsin Inhibitor from Glycine max (soybean) binds with the active site of trypsin enzyme, in a competitive inhibition manner.
Definição da unidade
One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.
Nota de preparo
Further purification of T9128 yielding an electrophoretically pure Kunitz inhibitor with increased activity.
Trypsin inhibitor is soluble in water and phosphate buffers at concentrations of 10 mg/ml or higher. Solutions at higher concentrations may be hazy and have a yellow to amber color.
Nota de análise
One mg will inhibit ≥1.0 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.
Outras notas
View more information on Trypsin Inhibitor.
Aplicação
Nº do produto
Descrição
Preços
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Resp. Sens. 1 - Skin Sens. 1
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Protocolos
This technical article described the Enzymatic Assay of Trypsin Inhibitor.
Chromatograms
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