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T9253

Sigma-Aldrich

Trypsin inhibitor

powder

Sinônimo(s):

Ovomucoid

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About This Item

Número CAS:
9035-81-8
Número CE:
232-906-9
Número MDL:
MFCD00082102
Código UNSPSC:
12352202
NACRES:
NA.77

product name

Trypsin inhibitor from chicken egg white, Type II-O, Partially purified ovomucoid, containing ovoinhibitor

fonte biológica

chicken egg white

Nível de qualidade

200

tipo

Type II-O

forma

powder

peso molecular

14 kDa

solubilidade

water: soluble 10 g/L

temperatura de armazenamento

2-8°C

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Descrição geral

25% of ovomucoid comprises carbohydrate.
Ovomucoid or trypsin inhibitor is an abundant protein in most avian egg whites. The hen′s egg protein is composed of about 186 amino acid residues, and is highly glycosylated. Three tandem domains are each homologous with pancreatic trypsin inhibitor. It is highly immunogenic, and probably accounts for most cases of egg allergy.

Aplicação

Trypsin inhibitor from chicken egg white has been used:
  • to produce denatured, oxidized and deglycosylated ovomucoid and used to stimulate patient derived cell cultures for mapping T cell epitopes
  • to treat nuclei for flow cytometric analysis
  • in Holtfreter′s solution, used for the dissociation of planarians into cells

Definição da unidade

One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.

Nota de análise

One mg will inhibit 0.8-1.6 mg of tryspin with activity of approx. 10,000 BAEE units per mg protein. May inhibit ≤0.3 mg of chymotrypsin with activity of approx. 40 BTEE units per mg protein.

Outras notas

Ovomucoid, from chicken egg white, does not itself inhibit chymotrypsin. Chymotrypsin inhibition is a measure of ovoinhibitor contamination by method of Feeney, R. E., et al., J. Biol. Chem., 238, 1415 (1963).
View more information on Trypsin Inhibitor.

Aplicação

Nº do produto
Descrição
Preços

Pictogramas

Health hazard
GHS08

Palavra indicadora

Danger

Frases de perigo

H317,H334

Classificações de perigo

Resp. Sens. 1 - Skin Sens. 1

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Novel B and T cell epitopes of chicken ovomucoid (Gal d 1) induce T cell secretion of IL-6, IL-13, and IFN-gamma
Holen E, et al.
Clinical and Experimental Allergy, 31(6), 952-964 (2001)
Expression of cyclin E in placentas with hydropic change and gestational trophoblastic diseases: implications for the malignant transformation of trophoblasts
Kim YT, et al.
Cancer, 89(3), 673-679 (2000)
Cultivation and characterization of planarian neuronal cells isolated by fluorescence activated cell sorting (FACS)
Asami M, et al.
Zoological Science, 19(11), 1257-1266 (2002)
Laura E Mickelsen et al.
eLife, 9 (2020-10-30)
The ventral posterior hypothalamus (VPH) is an anatomically complex brain region implicated in arousal, reproduction, energy balance, and memory processing. However, neuronal cell type diversity within the VPH is poorly understood, an impediment to deconstructing the roles of distinct VPH
Romain Cartoni et al.
PloS one, 12(9), e0184672-e0184672 (2017-09-20)
Improving axonal transport in the injured and diseased central nervous system has been proposed as a promising strategy to improve neuronal repair. However, the contribution of each cargo to the repair mechanism is unknown. DRG neurons globally increase axonal transport
Exibir todos os documentos relacionados

Artigos

Chymotrypsin

Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.

Protocolos

Enzymatic Assay of Trypsin Inhibitor

This technical article described the Enzymatic Assay of Trypsin Inhibitor.

Chromatograms

application for HPLC

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