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Sigma-Aldrich

Trypsin inhibitor

powder

Sinônimo(s):

Ovomucoid

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About This Item

Número CAS:
9035-81-8
Número CE:
232-906-9
Número MDL:
MFCD00082102
Código UNSPSC:
12352202
NACRES:
NA.77

product name

Trypsin inhibitor from chicken egg white, Type III-O (free of ovoinhibitor)

fonte biológica

chicken egg white

Nível de qualidade

300

tipo

Type III-O (free of ovoinhibitor)

forma

powder

peso molecular

14 kDa

solubilidade

water: soluble 10 g/L

temperatura de armazenamento

2-8°C

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Descrição geral

Ovomucoid or trypsin inhibitor is an abundant protein in most avian egg whites. The hen′s egg protein is composed of about 186 amino acid residues, and is highly glycosylated. Three tandem domains are each homologous with pancreatic trypsin inhibitor. It is highly immunogenic, and probably accounts for most cases of egg allergy.

Aplicação

Trypsin inhibitor from chicken egg white has been used:
  • to terminate proteolysis reaction
  • in OVO-1 solution to triturate the cell suspension for the dissociation of retina
  • in phosphate buffered saline for dissociation of cortices

Ações bioquímicas/fisiológicas

Ovomucoid possesses trypsin inhibitor activity. It has several conformational and linear epitopes that can be bound by immunoglobulin E (IgE). It is highly stable against heat and proteolysis.

Definição da unidade

One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.

Outras notas

View more information on Trypsin Inhibitor.

Aplicação

Nº do produto
Descrição
Preços

Pictogramas

Health hazard
GHS08

Palavra indicadora

Danger

Frases de perigo

H317,H334

Classificações de perigo

Resp. Sens. 1 - Skin Sens. 1

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Visite a Biblioteca de Documentos

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Jiang HF, et al.
Journal of virology, 91(12) (2017)
Meeyong Shin et al.
Allergy, asthma & immunology research, 5(1), 42-47 (2013-01-02)
It is known that ovomucoid, an egg allergen, is heat resistant and remains soluble after heating. However, a recent study showed that the antigenic activity of ovomucoid could be reduced by heating when egg white (EW) was mixed with wheat
Ovomucoid specific immunoglobulin E as a predictor of tolerance to cooked egg
Bartnikas LM, et al.
Allergy & rhinology (Providence, R.I.), 6(3), ar-2015 (2015)
Manipulation of the N-terminal sequence of the Borna disease virus X protein improves its mitochondrial targeting and neuroprotective potential
Ferree C, et al.
Faseb Journal (2015)
Immunopanning purification and long-term culture of human retinal ganglion cells
Zhang XM, et al.
Molecular Vision, 16, 2867-2867 (2010)
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Protocolos

Enzymatic Assay of Trypsin Inhibitor

This technical article described the Enzymatic Assay of Trypsin Inhibitor.

Chromatograms

application for HPLC

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