SAB4200719

Anti-Laminin antibody, Mouse monoclonal

clone LAM-89, purified from hybridoma cell culture

• Sinônimo(s): Anti-LAMA, Anti-LAMA1
• Código UNSPSC: 12352203
• NACRES: NA.41
• clone: LAM-89, monoclonal
• application: ELISA; EM; IF; IHC
• reatividade de espécies: feline, human, porcine
• técnica(s): ELISA: suitable; electron microscopy: suitable; immunoblotting: 0.03-0.06 μg/mL using Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane; immunofluorescence: suitable; immunohistochemistry: 20-40 μg/mL using pronase-retrieved formalin-fixed, paraffin-embedded human tongue sections.
• citations: 7

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 200
• forma do anticorpo: purified from hybridoma cell culture
• tipo de produto de anticorpo: primary antibodies
• clone: LAM-89, monoclonal
• Formulário: buffered aqueous solution
• peso molecular: ~850 kDa
• reatividade de espécies: feline, human, porcine
• validação aprimorada: independent; Learn more about Antibody Enhanced Validation
• concentração: ~1.0 mg/mL
• técnica(s): ELISA: suitable; electron microscopy: suitable; immunoblotting: 0.03-0.06 μg/mL using Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane; immunofluorescence: suitable; immunohistochemistry: 20-40 μg/mL using pronase-retrieved formalin-fixed, paraffin-embedded human tongue sections.
• Isotipo: IgG1
• nº de adesão UniProt: P25391
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: cat ... Lama1(101083299) ; human ... LAMA1(284217) ; pig ... Lama1(100625589)

Descrição

Descrição geral

Monoclonal anti-laminin antibody (mouse IgG1 isotype) is derived from the LAM-89 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified laminin from human placenta. Monoclonal anti-laminin antibody specifically recognizes laminin from human, feline and porcine origin. The antibody shows reactivity with laminin and specifically staining basal membrane of blood vessels, epithelium, nerve and muscle fiber. Monoclonal anti-laminin shows no reaction with collagen IV, fibronectin, vitronectin and chondroitin sulfate type A, B or C. Laminins are complexed extracellular glycoproteins which assemble the basal components of the basement membrane (BM). Each laminin is a heterotrimer (~850kDa) comprising of an α (~400kDa), β (~200kDa) and γ (~200kDa) chains all held together by disulfide bonds.

Especificidade

Monoclonal Anti-Laminin antibody specifically recognizes Laminin from human, feline and porcine origin.

Imunogênio

purified Laminin from human Placenta

Aplicação

Monoclonal Anti-Laminin is recommended to use in various immunochemical assays, including immunohistochemistry, immunoblotting (~850 kDa), immunofluorescence, Enzyme linked immunosorbent assay (ELISA) and electron microscopy.

Ações bioquímicas/fisiológicas

Laminins contribute to the structure of the extracellular matrix (ECM). It also plays a major role in the regulation of variety of cellular processes, such as the rates of cell proliferation, differentiation, adhesion, migration, cell shaping, phenotype stability and resistance to apoptosis. Laminin polymers forms supramolecular network with collagen IV polymers through heparan sulfate proteoglycan and by nidogen (entactin) and plays an important role in the basement membrane (BM) stability. In mouse, mutations in the gene is associated with the development of muscular dystrophy.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Armazenamento e estabilidade

Store at –20°C. For continuous use, store at 2–8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable