Pular para o conteúdo
Merck
Todas as fotos(1)

Documentos Principais

Visualizar Toda a Documentação

P2645

Sigma-Aldrich

Protein Kinase A Catalytic Subunit from bovine heart

≥9 units/μg protein (cyclic-AMP is not required for this activity), lyophilized (white powder to sticky mass to hard pellet)

Sinônimo(s):

PKA

Faça loginpara ver os preços organizacionais e de contrato
Todas as fotos(1)

About This Item

Número da licença da enzima:
2.7.11.11 (BRENDA, IUBMB)
Número MDL:
MFCD00132114
Código UNSPSC:
12352204
eCl@ss:
32160410
NACRES:
NA.32
Preço e disponibilidade não estão disponíveis no momento.

Formulário

lyophilized (white powder to sticky mass to hard pellet)

Nível de qualidade

200

atividade específica

≥9 units/μg protein (cyclic-AMP is not required for this activity)

peso molecular

40,862 Da

temperatura de armazenamento

−20°C

Descrição geral

Protein Kinase A enzyme is composed of two subunits- catalytic and regulatory. The catalytic subunit exists as a monomer in the presence of cAMP and has a molecular weight of 40,862 Da.[1][2]

Aplicação

Protein Kinase A (PKA) Catalytic Subunit from bovine heart has been used-
  • to study PKA-mediated inhibition of IRK1 (inwardly rectifying K+) channels[3]
  • in in Vitro PKA pPhosphorylation assay[4]
  • in Vitro affinity binding assays[5]
  • to study effects of PKA on inspiratory drive currents in functionally active motorneurons[6]

Ações bioquímicas/fisiológicas

Protein Kinase A (PKA) controls the transduction of Hedgehog signaling and participates in proliferation and fate specification.[7] It phosphorylates several neurotransmitter receptors, transcription factors and constituents of various intracellular signaling pathways.[8]
Protein Kinase A catalyzes the transfer of terminal phosphate from ATP to threonine or serine residues present on various proteins. This protein is inactive in the absence of cAMP, where the catalytic and regulatory subunits are bound together. The regulatory subunit, in the presence of cAMP, binds to cAMP and releases the catalytic subunit.[9]

Embalagem

Package size based on phosphorylating units

Definição da unidade

Phosphorylating Activity: One unit will transfer 1.0 picomole phosphate from ATP to hydrolyzed and partially dephosphorylated casein per minute at pH 6.5 at 30°C, determined by measuring the production of ADP.

forma física

Lyophilized powder with sucrose and phosphate buffer salts as stabilizer.

Nota de preparo

Prepared from protein kinase A (P 5511)

Exoneração de responsabilidade

Please note that the pack size has been changed to align with the unit definition, while the number of phosphorylating units remain the same as before.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Escolha uma das versões mais recentes:

Certificados de análise (COA)

Lot/Batch Number
0000303135
0000344877
110M7720
021M7676
031M7674

Não está vendo a versão correta?

Se precisar de uma versão específica, você pode procurar um certificado específico pelo número do lote ou da remessa.

Pesquise por COA

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Christopher M Bocchiaro et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 23(4), 1099-1103 (2003-02-25)
Plasticity underlying adaptive, long-term changes in breathing behavior is hypothesized to be attributable to the modulation of respiratory motoneurons by intracellular second-messenger cascades. In quiescent preparations, protein kinases, including cAMP-dependent protein kinase A (PKA), potentiate glutamatergic inputs. However, the dynamic
Regions of the Escherichia coli primary sigma factor sigma70 that are involved in interaction with RNA polymerase core enzyme.
Nagai, et al.
Genes Cells, 2, 725-734 (2019)
Petr G Vikhorev et al.
Cardiovascular research (2020-11-03)
Dilated cardiomyopathy (DCM) is associated with mutations in many genes encoding sarcomere proteins. Truncating mutations in the titin gene TTN are the most frequent. Proteomic and functional characterisations are required to elucidate the origin of the disease and the pathogenic
J A Byun et al.
Science advances, 6(25), eabb1250-eabb1250 (2020-07-01)
The functional response of a signaling system to an allosteric stimulus often depends on subcellular conditions, a phenomenon known as pluripotent allostery. For example, a single allosteric modulator, Rp-cAMPS, of the prototypical protein kinase A (PKA) switches from antagonist to
Protein kinase A activity and Hedgehog signaling pathway
Vitamins & Hormones, 88, 273-291 (2012)
Exibir todos os documentos relacionados

Questions

Reviews

No rating value

Active Filters

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica