P5511
Protein Kinase A from bovine heart
≥0.8 units/μg protein, lyophilized powder
Sinônimo(s):
Protein Kinase, 3′,5′-cyclic-AMP-dependent from bovine heart
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About This Item
Número CAS:
Número MDL:
Código UNSPSC:
12352204
eCl@ss:
32160410
NACRES:
NA.77
Produtos recomendados
forma
lyophilized powder
atividade específica
≥0.8 units/μg protein
composição
Protein, ≥70%
temperatura de armazenamento
−20°C
Informações sobre genes
cow ... PRKACA(282322) , PRKACB(282323)
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Descrição geral
Protein Kinase A (PKA) is a serine/threonine kinase, which exists as a tetrameric holoenzyme.
Aplicação
Protein Kinase A from bovine heart has been used to determine its activity using a molecular beacon probe. It has also been used to study the interaction of staurosporine with the ATP-binding site of kinases.
Protein Kinase A from bovine heart has been used to inhibit the phosphorylation of myosin.
Ações bioquímicas/fisiológicas
Protein Kinase A (PKA) inhibits hormone-sensitive lipase translocation from cytosol to storage droplets and blocks lipolysis. It regulates apoptosis, mitochondrial respiration and ATP synthesis. PKA is modulated by protease calpain.
Many 3′,5′-cyclic AMP dependent protein kinases have been reported. Structural studies (Traugh, J.A., et al., Meth. Enzymol., 38, 290 [1974]) show the presence of at least two subunits, the regulatory subunit and the catalytic subunit. When both units are linked together, the catalytic activity is inhibited. However, when the cyclic-AMP binds to the regulatory subunit, the catalytic subunit is released and can then catalyze the transfer of phosphate from ATP to various proteins.
Embalagem
Package size based on protein content
Definição da unidade
One unit will transfer 1.0 picomole phosphate from ATP to hydrolyzed and partially dephosphorylated casein per minute at pH 6.5 at 30°C in the presence of cyclic AMP, determined by measuring the production of ADP.
forma física
Crude lyophilized powder containing EDTA and potassium phosphate, pH 7.5.
Nota de preparo
Fractionated essentially by procedure of Gilman, A., Proc. Natl. Acad. Sci. USA, 67, 305 (1970).
Nota de análise
Protein determined by biuret.
inibidor
Nº do produto
Descrição
Preços
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
Certificados de análise (COA)
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