539481-M

PKA, Catalytic Subunit, Mouse, Recombinant, E. coli

• Sinônimo(s): cAMP-Dependent Protein Kinase, Catalytic Subunit, Protein Kinase A, Catalytic Subunit
• Número da licença da enzima: 2.7.1.37 (BRENDA, IUBMB)
• Código UNSPSC: 12352202

Propriedades

• forma: liquid
• Nível de qualidade: 100
• atividade específica: ≥2.5 x 10⁶ U/mL
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; avoid repeated freeze/thaw cycles
• atividade externa: Phosphatases, proteases, none detected
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Recombinant, mouse PKA catalytic subunit expressed in E. coli. Regulates a number of cellular functions including gene expression, carbohydrate metabolism, Ca²⁺ homeostasis, and ion channel function. Suitable for studying the phosphorylation of purified substrates and for use with cell extracts. Unlike the holoenzyme, the catalytic subunit does not require cAMP for activation.

Recombinant, mouse PKA catalytic subunit expressed in E. coli. The catalytic subunit of protein kinase A that combines with the regulatory subunit, in the absence of cAMP, to form the inactive PKA holoenzyme. Unlike the holoenzyme, the catalytic subunit does not require cAMP for activation. PKA-Cα is reported to potentiate IP₃-induced Ca²⁺ mobilization in isolated hepatocytes. Suitable for studies of PKA phosphorylation in vitro with purified substrates or following the introduction into intact cells or cell extracts.

Advertência

Toxicity: Standard Handling (A)

Definição da unidade

One unit is defined as the amount of enzyme that will transfer 1.0 pmol phosphate to Kemptide (LRRASLG; Cat. No. 05-23-4900) per min at 30°C, pH 7.5.

forma física

In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, 2 mM DTT, 50% glycerol, pH 7.5.

Reconstituição

Following initial thaw, aliquot and freeze (-20°C).

Outras notas

Grant, B.D., and Adams, J.A. 1996. Biochemistry35, 2022.
Lee, S-L. and Steinberg, R.A. 1996. J. Biol. Chem.271, 16553.
Adams, J.A., et al. 1995. Biochemistry 34, 2447.
Bosma, M.M., et al. 1993. J. Neurosci.13, 5242.
Brindle, P., et al. 1993. Nature364, 821.
Hajnoczky, G., et al. 1993. Biochem. J.293, 413.
Sculptoreanu, A., et al. 1993. Proc. Natl. Acad. Sci. USA90, 10135.
Wang, L-Y., et al. 1993. Science259, 1173.
Slice, L.W., and Taylor, S.S. 1989. J. Biol. Chem.264, 20940.
Uhler, M.D., et al. 1986. Proc. Natl. Acad. Sci. USA83, 1300.
Walsh, D.A., et al. 1968. J. Biol. Chem.243, 3763.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable