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P0001

Sigma-Aldrich

Phosphatase Inhibitor Cocktail

ReadyShield® ready-to-use solution, for the inhibition of Serine/Threonine Protein Phosphatases and L-Isozymes of Alkaline Phosphatase

Sinônimo(s):

Phosphatase inhibitor cocktail

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.54
Formulário:
ready-to-use solution
temperatura de armazenamento:
−20°C

Nome do produto

ReadyShield® Phosphatase Inhibitor Cocktail, For Serine/Threonine Protein Phosphatases and L-Isozymes of Alkaline Phosphatase

Nível de qualidade

200

Formulário

ready-to-use solution

embalagem

pkg of 1 mL (in amber polypropylene vial)
pkg of 5 mL (in amber polypropylene vial)

temperatura de armazenamento

−20°C

Categorias relacionadas

Descrição geral

This phosphatase Inhibitor Cocktail contains inhibitors with a broad specificity for L-isozymes of alkaline phosphatases, and serine/ threonine protein phosphatases. Phosphatase Inhibitor Cocktail has been tested on extracts from various animal tissues such as human placenta and bovine liver, and on extracts of A431, CHO, and U937 cells.

This cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation.
ReadyShield® P0001 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail P0044.

Características e benefícios

  • Does not freeze in storage for quick, convenient use.
  • Specific for Serine/Threonine Protein Phosphatases and L-Isozymes of Alkaline Phosphatase.
  • Suggested as 100 X solution.
  • Reclosable screw-top vials.
  • Can be stored in cooler for approx. 1 month or room temperature for a week.

Nota de preparo

This cocktail is supplied as a clear solution in dimethyl sulfoxide. It is recommended to dilute the cocktail at 1 mL of cocktail per 500 mg of protein extracted from tissue or cells. Often a final concentration of 1% (v/v) is used, which will inhibit phosphatase activities found in the 27,000 x g supernatant from human placenta and bovine liver, and in the 100,000 x g supernatant from A431, CHO, and U937 extracts.

Outras notas

For life science research only. Not for use in diagnostic procedures.

Informações legais

ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Certificados de análise (COA)

Lot/Batch Number
0000344603
0000344603
0000247688
0000247690
0000219669

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Artigos

ReadyShield Phosphatase and Protease Inhibitor Cocktail FAQ

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

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