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Merck

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PPC2020

Protease and Phosphatase Inhibitor Cocktail

ReadyShield® ready-to-use solution, for the inhibition of serine, cysteine, acid proteases, aminopeptidases, L-isozymes of alkaline phosphatases, non-freezing solution

Sinônimo(s):

Protease and phosphatase inhibitor cocktail, inhibitor cocktail

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Tamanho/SKUDisponibilidadePreço
1 mL
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R$ 2.594,00
5 mL
Verificar disponibilidade no carrinho
R$ 5.507,00

Sobre este item

UNSPSC Code:
12352200
NACRES:
NA.54
Form:
ready-to-use solution
Solubility:
water: soluble
Storage temp.:
−20°C

R$ 2.594,00


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Nome do produto

ReadyShield® Protease and Phosphatase Inhibitor Cocktail, non-freezing solution

Quality Segment

form

ready-to-use solution

packaging

pkg of 1 mL (in amber polypropylene vial), pkg of 5 mL (in amber polypropylene vial)

input

mammalian sample(s)

solubility

water: soluble

storage temp.

−20°C

General description

Protease and Phosphatase Inhibitor Cocktail has been optimized and tested for mammalian cellular and tissue extracts. It contains inhibitors with a broad specificity for serine, cysteine, acid proteases, aminopeptidases, L-isozymes of alkaline phosphatases, and serine/ threonine protein phosphatases.

Protease and Phosphatase Inhibitor Cocktail has been tested on extracts from various animal tissues such as human placenta and bovine liver, and on extracts of A431, CHO, and U937 cells.

The cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation.
ReadyShield® PPC2020 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail PPC1010

Features and Benefits

  • Does not freeze in storage for quick, convenient use.
  • For broad ranging inhibition of both proteases and phosphatases.
  • Suggested as 100 X solution.
  • Reclosable screw-top vials.
  • Can be stored in cooler for approx. 1 month or room temperature for a week.

Preparation Note

The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 500 mg of protein extracted from tissue or cells. In many cases, the cocktail can be used at a final concentration of 1% (v/v), 1 ml of cocktail solution per 100 ml of extraction buffer.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany

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Este Item
P0001PPC1010P8215
description

non-freezing solution

description

For Serine/Threonine Protein Phosphatases and L-Isozymes of Alkaline Phosphatase

description

-

description

for use with fungal and yeast extracts, DMSO solution

form

ready-to-use solution

form

ready-to-use solution

form

DMSO solution

form

DMSO solution

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

solubility

water: soluble

solubility

water: soluble

solubility

water: soluble

solubility

water: soluble

packaging

pkg of 1 mL (in amber polypropylene vial), pkg of 5 mL (in amber polypropylene vial)

packaging

pkg of 5 mL (in amber polypropylene vial), pkg of 1 mL (in amber polypropylene vial)

packaging

pkg of 1 ml, 5 ml ×

packaging

-

input

mammalian sample(s)

input

mammalian sample(s)

input

general use sample(s)

input

plant, fungi and insect sample(s)


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pictograms

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signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Classe de armazenamento

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

1–3 of 3 Questions  
  1. What is the recommended storage temperature for ReadyShield® products?

    1 answer
    1. The product is stable at -20 °C for 2 years from the assay date, which can be found on the lot specific CoA. The solution will not freeze in -20 °C storage and can be used directly from the freezer. Our experts have collected additional stability information that show ReadyShield® products are stable for at least one month at 4 °C, or for at least one week at 25 °C (room temperature).

      Helpful?

  2. Do ReadyShield® inhibitor cocktails include EDTA?

    1 answer
    1. No, all the cocktails are EDTA-free. Alternative metalloprotease inhibitors are included in the cocktail when needed, depending on the sample type. EDTA can be added if it does not interfere with the downstream purification or activity of your protein of interest. Please explore the reagent offerings below for recommended, ready-to-use EDTA products.

      Helpful?

  3. Does PPC2020 contain EDTA?

    1 answer
    1. PPC2020 does not contain EDTA

      Helpful?

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