Pular para o conteúdo
Merck
Todas as fotos(1)

Documentos Principais

Visualizar Toda a Documentação

N1891

Sigma-Aldrich

p-Nitrophenyl phosphate

SIGMAFAST™, chromogenic, tablet

Sinônimo(s):

pNPP tablets

Faça loginpara ver os preços organizacionais e de contrato
Todas as fotos(1)

About This Item

Número MDL:
MFCD00284586
Código UNSPSC:
12352204
NACRES:
NA.83
Preço e disponibilidade não estão disponíveis no momento.

Nome do produto

SIGMAFAST™ p-Nitrophenyl phosphate Tablets, tablet, To prepare 5 mL

Formulário

tablet

solubilidade

deionized water: soluble 5 mL

temperatura de armazenamento

−20°C

Procurando produtos similares? Visita Guia de comparação de produtos

Categorias relacionadas

Aplicação

Developed for use as a soluble substrate for the detection of alkaline phosphatase activity in Enzyme Immunoassays (EIA). pNPP is the substrate of choice in high sensitivity alkaline phosphatase-based EIA assays.One pNPP tablet and one Tris buffer tablet are dissolved in deionized water and provide a ready-to-use substrate. Each SIGMAFAST pNPP tablet set yields a solution containing 1.0 mg/ml pNPP, 0.2 M Tris buffer. EIA applications using pNPP may be read in timed assays or stopped with 3N NaOH for delayed readings. SIGMAFAST™ require only the addition of distilled or deionized water to prepare an active substrate solution. Tablets are individually packaged in foil packets.
SIGMA FAST p-Nitrophenyl phosphate Tablets has been used:
  • to quantify non-secreted alkaline phosphatase to monitor differentiation of osteoblast-like cells (SaoS-2)[1]
  • to stain murine preosteoblast MC3T3-E1 cells in alkaline phosphatase assay[2]
  • as a substrate for ExtrAvidin® alkaline phosphatase to measure hemoglobin-bound α-synuclein using enzyme-linked immunosorbent assay (ELISA)[3]
  • as a substrate for alkaline phosphatase conjugated antibodies for detection in ELISA of abirin toxin[4]

Reconstituição

Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing pNPP.

Informações legais

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Escolha uma das versões mais recentes:

Certificados de análise (COA)

Lot/Batch Number
0000336007
SLCQ9495
0000336006
0000336007
0000336006

Não está vendo a versão correta?

Se precisar de uma versão específica, você pode procurar um certificado específico pelo número do lote ou da remessa.

Pesquise por COA

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Ruklanthi de Alwis et al.
The Journal of infectious diseases, 219(2), 295-304 (2018-10-16)
Nontyphoidal Salmonella (NTS) organisms are a major cause of gastroenteritis and bacteremia, but little is known about maternally acquired immunity and natural exposure in infant populations residing in areas where NTS disease is highly endemic. We recruited 503 pregnant mothers
Ronit Rosenfeld et al.
Nature communications, 12(1), 944-944 (2021-02-13)
The coronavirus disease 2019 (COVID-19) pandemic, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), exhibits high levels of mortality and morbidity and has dramatic consequences on human life, sociality and global economy. Neutralizing antibodies constitute a highly promising
Conall H Watson et al.
PLoS neglected tropical diseases, 11(7), e0005786-e0005786 (2017-07-21)
Fiji, an upper-middle income state in the Pacific Ocean, has experienced an increase in confirmed case notifications of enteric fever caused by Salmonella enterica serovar Typhi (S. Typhi). To characterize the epidemiology of typhoid exposure, we conducted a cross-sectional sero-epidemiological
Zimu Wu et al.
International journal of clinical and experimental pathology, 12(5), 1757-1763 (2020-01-15)
Post-stroke induction of alpha-synuclein (AS), a neuronal protein implicated in the pathogenesis of Parkinson's disease (PD), has been demonstrated to induce secondary brain damage after cerebral ischemia. Therefore, understanding the expression and pathogenic modifications of AS is clinically meaningful for
Yuankun Zhai et al.
Journal of cellular physiology, 234(10), 18602-18614 (2019-03-27)
Homocysteine (Hcy) is detrimental to bone health in a mouse model of diet-induced hyperhomocysteinemia (HHcy). However, little is known about Hcy-mediated osteoblast dysfunction via mitochondrial oxidative damage. Hydrogen sulfide (H2 S) has potent antioxidant, anti-inflammatory, and antiapoptotic effects. In this
Exibir todos os documentos relacionados

Artigos

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Protocolos

ELISA Procedures

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

Questions

Reviews

No rating value

Active Filters

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica