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P7998

Sigma-Aldrich

Alkaline Phosphatase Yellow

alkaline phosphatase substrate, chromogenic liquid

Sinônimo(s):

Alkaline phosphatase substrate solution (pNPP)

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100 ML
R$ 995,00

R$ 995,00


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100 ML
R$ 995,00

About This Item

Código UNSPSC:
12352204
eCl@ss:
42010105
NACRES:
NA.83

R$ 995,00


Em estoqueDetalhes


Nome do produto

Alkaline Phosphatase Yellow (pNPP) Liquid Substrate System for ELISA, ready to use solution

Nível de qualidade

Formulário

liquid

temperatura de armazenamento

2-8°C

Descrição geral

The product is ready to use buffered alkaline phosphatase substrate consist of p-nitrophenylphosphate (pNPP). This is the advanced substrate system contains insoluble stabilizer that enhances the stability and reactivity as well as reduces the base-line absorption. It produces a soluble end product, hence not recommended for blotting or histochemistry applications.

Aplicação

Develops a soluble yellow reaction product that may be read at 405 nm. For endpoint assays, the reaction may be stopped with 3 N NaOH. Recommended for ELISA (microwell) procedures; not recommended for membrane applications.

Atenção

 Stable at least one year at 2-8 °C.

forma física

Ready to use

Nota de preparo

Contains p-nitrophenyl phosphate, a substrate for alkaline phosphatase.

Pictogramas

Health hazardCorrosion

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Eye Dam. 1 - Skin Irrit. 2 - STOT RE 2

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Cristina A Teixeira et al.
Clinical science (London, England : 1979), 130(18), 1665-1675 (2016-07-08)
Transthyretin (TTR)-related amyloidoses are diseases characterized by extracellular deposition of amyloid fibrils and aggregates in tissues composed of insoluble misfolded TTR that becomes toxic. Previous studies have demonstrated the ability of small compounds in preventing and reversing TTR V30M deposition
Laura C Jacques et al.
Nature communications, 11(1), 1892-1892 (2020-04-22)
Streptococcus pneumoniae serotype 1 is the predominant cause of invasive pneumococcal disease in sub-Saharan Africa, but the mechanism behind its increased invasiveness is not well understood. Here, we use mouse models of lung infection to identify virulence factors associated with
Hannah M Rowe et al.
Infection and immunity, 87(10) (2019-07-17)
Acute otitis media is one of the most common childhood infections worldwide. Currently licensed vaccines against the common otopathogen Streptococcus pneumoniae target the bacterial capsular polysaccharide and confer no protection against nonencapsulated strains or capsular types outside vaccine coverage. Mucosal
Baoxin Wang et al.
Experimental and therapeutic medicine, 13(1), 178-186 (2017-01-27)
Damage to the recurrent laryngeal nerve often causes hoarseness, dyspnea, dysphagia, and sometimes asphyxia due to vocal cord paralysis which result in a reduction of quality of life. Brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) play
Ortal Danino et al.
Rheumatology (Oxford, England), 57(8), 1472-1480 (2018-04-25)
Calcium pyrophosphate deposition (CPPD) is associated with osteoarthritis and is the cause of a common inflammatory articular disease. Ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (eNPP1) is the major ecto-pyrophosphatase in chondrocytes and cartilage-derived matrix vesicles (MVs). Thus, eNPP1 is a principle contributor to

Artigos

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Questions

  1. Can this be used after it has been frozen?

    1 answer
    1. The stability of this solution has not been evaluated at −20°C. The freeze/thaw process may contribute to decomposition of the substrate solution. Signs of degradation include changes in color or turbidity and precipitation of the pNPP.

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