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M7403

Sigma-Aldrich

MCDB 153 Medium

With trace elements, L-glutamine and 28mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

Sinônimo(s):

Molecular, Cellular, and Development Biology 153 medium

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.75

Nível de qualidade

500

forma

powder

técnica(s)

cell culture | mammalian: suitable

componentes

NaHCO3: no
L-glutamine: 0.8772 g/L
glucose: 1.081 g/L (Dextro)
sodium pyruvate: 0.055 g/L
phenol red: 0.001242 g/L
HEPES: 6.6 g/L (25mM)

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

Categorias relacionadas

Descrição geral

MCDB 153 media are modifications of Ham′s nutrient mixture F-12, designed for the growth of human keratinocytes, clonal growth of chicken embryo fibroblasts, and Chinese hamster ovary (CHO) cells using low levels of fetal bovine serum protein (FBSP), extensive trace elements or no serum protein.

Aplicação

MCDB 153 Medium has been used as a component of melanoma isolation media (MIM) to culture human non-metastatic and metastatic melanoma cells.

Quantidade

Formulated to contain 17.6 grams of powder per liter of medium.

Reconstituição

Supplement with 1.18 g/L sodium bicarbonate.

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suplemento

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Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

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Protocolos

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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