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R8755

Sigma-Aldrich

Meio RPMI-1640

With ʟ-glutamine, without phenol red and sodium bicarbonate, powder, suitable for cell culture

Sinônimo(s):

Roswell Park Memorial Institute 1640 medium

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About This Item

Número MDL:
Código UNSPSC:
12352207
NACRES:
NA.75

product name

Meio RPMI-1640, Modified, with L-glutamine, without phenol red and sodium bicarbonate, powder, suitable for cell culture

forma

powder

técnica(s)

cell culture | mammalian: suitable

componentes

HEPES: no
phenol red: no
L-glutamine: yes
NaHCO3: no
sodium pyruvate: no

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

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Descrição geral

O meio RPMI 1640 foi desenvolvido no Instituto Roswell Park Memorial, em 1966, por Moore e seus colaboradores. Uma modificação do meio 5A de McCoy. Ele foi formulado para dar suporte a células linfoblastoides em cultura de suspensão, mas desde então demonstrou ser capaz de fornecer suporte a uma ampla gama de células dependentes de ancoragem. Destinado originalmente para ser usado com um suplemento de soro, demonstrou-se que o RPMI 1640 dá suporte a diversas linhagens celulares na ausência de soro. Ele também foi usado amplamente nos protocolos de fusão e no crescimento de células híbridas. Esse meio é adequado para a cultura de leucócitos humanos normais e neoplásicos.

Aplicação

RPMI-1640 Medium is used for the following applications:
  • Used in cell culture medium: Murine thymoma EL-4 cells were cultured in RPMI 1640 medium supplemented with other components
  • Used for the mycobacterial infection of splenocytes (in antibiotic-free RPMI 1640 medium)
  • Used for cell isolation and culture
  • Used in culture medium (as one of the component) during lymphocyte separation and culture, ELISPOT Assay, Ex Vivo Proliferation Assay
  • Used in medium for gp39 gene expression and CD40-Immunoglobuin binding assays
  • Used for the preparation of antifungal agents

Quantidade

Formulated to contain 10.4 grams of powder per liter of medium.

Reconstituição

Supplement with 2.0 g/L sodium bicarbonate.

Outras notas

Phenol red has been shown to interfere with the growth of some cells at clonal densities. Use this medium when working with stem cells or when growing cells at low densities. Also recommended for in vitro diagnostics use.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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Artigos

ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.

Protocolos

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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