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L0543
LB Agar Kanamycin-50, Plates
pre-poured LB agar plates with 50 ug/ml kanamycin
Sinônimo(s):
kanamycin agar plates
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About This Item
Código UNSPSC:
41121800
esterilidade:
sterile; autoclaved
adequação:
selective for Escherichia coli
selective for coliforms
selective for coliforms
Preço e disponibilidade não estão disponíveis no momento.
Produtos recomendados
grau
for molecular biology
Nível de qualidade
esterilidade
sterile; autoclaved
Formulário
plates (10 cm)
plates (10cm, 20-25ml each)
capacidade
20-25 mL
temperatura de armazenamento
2-8°C
adequação
selective for Escherichia coli
selective for coliforms
Descrição geral
Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.
Aplicação
Suitable for selective cultivation of E. coli strains that contain plasmids conferring kanamycin resistance. The bacteria can be used for DNA plasmid production or production of recombinant proteins. LB Agar Kanamycin-50, Plates has been used in the screening of Agrobacterium tumefaciens[1] and E.coli JM83 strain with pGD103 plasmid.[2]
Características e benefícios
Miller′s LB Agar plates provide:
- Convenient, ready-to-use 10cm format
- Standard formulation plus antibiotic
Componentes
15g/L Agar
10g/L Tryptone
10g/L NaCl
5g/L Yeast Extract
50μg/ml kanamycin
10g/L Tryptone
10g/L NaCl
5g/L Yeast Extract
50μg/ml kanamycin
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Nº do produto
Descrição
Preços
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Artigos
Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.
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What is the Department of Transportation shipping information for this product?
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Why do the LB-agar plates with antibiotics have such a short expiration date?
1 answer-
The antibiotics in the LB-agar plates are not stable. The antibiotic degrades over time, making it ineffective at selecting transformed bacteria grown on the plate. Because of this, we recommend the plates are used as soon as possible once they are received. For labs with low use, the plates may expire quickly, so they may benefit from pouring their own plates using the LB - Miller powder with agar formulation (L3147).
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