K0254

Kanamycin solution from Streptomyces kanamyceticus

liquid, suitable for cell culture and plant cell culture, BioReagent

• Sinônimo(s): Kanamycin A
• Número CAS: 25389-94-0
• Número MDL: MFCD00070289
• Código UNSPSC: 12352207
• ID de substância PubChem: 329817120
• NACRES: NA.76

Propriedades

• product name: Kanamycin solution from Streptomyces kanamyceticus, 50 - 60 mg/mL in 0.9% NaCl, BioReagent, liquid, 0.1 μm filtered, suitable for cell culture
• fonte biológica: Streptomyces kanamyceticus
• esterilidade: 0.1 μm filtered
• linha de produto: BioReagent
• forma: liquid
• concentração: 50 - 60 mg/mL in 0.9% NaCl
• técnica(s): cell culture | mammalian: suitable; cell culture | plant: suitable
• Impurezas: endotoxin, tested
• espectro de atividade do antibiótico: Gram-negative bacteria; Gram-positive bacteria; mycobacteria; mycoplasma
• Modo de ação: protein synthesis | interferes
• temperatura de armazenamento: 2-8°C
• cadeia de caracteres SMILES: NC[C@H]1O[C@H](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
• InChI: 1S/C18H36N4O11/c19-2-6-10(25)12(27)13(28)18(30-6)33-16-5(21)1-4(20)15(14(16)29)32-17-11(26)8(22)9(24)7(3-23)31-17/h4-18,23-29H,1-3,19-22H2/t4-,5+,6-,7-,8+,9-,10-,11-,12+,13-,14-,15+,16-,17-,18-/m1/s1
• chave InChI: SBUJHOSQTJFQJX-NOAMYHISSA-N

Descrição

Descrição geral

Kanamycin sulfate is a broad spectrum aminoglycoside-antibiotic derived from Streptomyces kanamyceticus. It is used as an additive in culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. Kanamycin sulfate can also be used as a selection agent for cells transformed with kanamycin B resistance gene. It is recommended for use in cell culture applications at 2 mL/L.

Aplicação

Kanamycin solution from Streptomyces kanamyceticus has been used:

• to induce deafness in neonatal rats
• as an endothelial growth medium component for culturing human umbilical vein endothelial cells (HUVECs)
• as medium component for Mycobacteria Cultures and Top10 E coli cells

Ações bioquímicas/fisiológicas

Mode of Action: The product acts by binding to the 70S ribosomal subunit, inhibiting translocation and eliciting miscoding.

Mode of Resistance: Aminoglycoside-modifying enzymes (including acetyltransferase, phosphotransferase, nucleotidyltransferase) can alter this antibiotic, preventing its interaction with ribosomes.

Antimicrobial spectrum: Kanamycin sulfate is effective against gram-negative and gram-postiive bacteria, and mycoplasma.

Atenção

Solutions are stable at 37°C for approximately 5 days. Aqueous stock solutions can be stored at 2-8°C for long term storage.

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H360D
• Declarações de precaução: P201 - P308 + P313
• Classificações de perigo: Repr. 1B
• Código de classe de armazenamento: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable