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L2542

Sigma-Aldrich

LB Broth (Miller)

Liquid microbial growth medium

Sinônimo(s):

Luria Broth Miller, Luria-Bertani broth, Miller

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About This Item

Código UNSPSC:
41106200
NACRES:
NA.85

grau

for molecular biology

esterilidade

sterile; 0.2 μm filtered

forma

liquid

técnica(s)

microbiological culture: suitable

pH

6.5-7.2

aplicação(ões)

microbiology

temperatura de armazenamento

room temp

adequação

nonselective for Escherichia coli
nonselective for coliforms

Categorias relacionadas

Descrição geral

Miller’s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. It enables fast growth, improves growth yields for various bacterial species, and can retain the hydrophilic behavior of samples for longer periods.

Aplicação

LBBroth (Miller) is suitable for:

  • non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
  • selective cultivation when appropriate antibiotics are added.
  • the cultivation of E-coli cells for anti-bacterial assays.

Características e benefícios

Miller′s LB liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

Componentes

10g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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