D9281

Lambda DNA EcoR I Hind III Digest

for DNA electrophoresis

• Código UNSPSC: 41141827
• NACRES: NA.25

Propriedades

• Nível de qualidade: 100; 200
• Formulário: liquid
• uso: 100 uses (0.1 mg size); 500 uses (0.5 mg size)
• adequação: suitable for electrophoresis (DNA)
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Sigma′s Lambda DNA EcoR I Hind III Digest contains 13 fragments from 125 – 21,226 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Aplicação

Lambda DNA EcoR I Hind III Digest has been used as DNA markers for comparing the molecular weights of polymerase chain reaction (PCR) amplified products.[1]

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

Componentes

Sigma′s Lambda DNA EcoR I Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Outras notas

Contains 13 fragments, 125–21,226 bp

For optimal resolution, the recommended agarose gel concentration is 0.7%.

Nota de preparo

Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.

Informações de segurança

• Pictogramas: GHS07
• Palavra indicadora: Warning
• Frases de perigo: H317
• Declarações de precaução: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Classificações de perigo: Skin Sens. 1
• Código de classe de armazenamento: 13 - Non Combustible Solids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable