D0672

φX174 DNA Marker Hae III Digest

for DNA electrophoresis

• Código UNSPSC: 41105335
• NACRES: NA.25

Propriedades

• Formulário: liquid
• uso: 200 uses (0.1 mg size); 40 uses (20 μg size)
• adequação: suitable for electrophoresis (DNA)
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Sigma′s Phi X174 DNA Marker Hae III Digest contains 11 fragments from 72 − 1,353 bp. Five ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Componentes

Sigma′s Phi X174 DNA Marker Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Nota de preparo

φX174RF-I DNA was digested with Hae III to completion, then purified by phenol extraction.

Outras notas

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)