C7555

Concanavalin A from Canavalia ensiformis (Jack bean)

agarose conjugate, Type VI, saline suspension

• Sinônimo(s): ConA
• Código UNSPSC: 12161501
• NACRES: NA.32

Propriedades

• fonte biológica: Canavalia ensiformis
• conjugado: agarose conjugate
• tipo: Type VI
• Formulário: saline suspension
• Extensão da rotulagem: 15-30 mg concanavalin A protein per mL
• Matriz: cross-linked 4% beaded agarose
• ativação da matriz: cyanogen bromide
• ligação da matriz: amino
• espaçador de matriz: 1 atom
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Concanavalin A (Con A), isolated from Jack beans (Canavalia ensiformis), is a mannose or glucose specific-binding lectin.[1] The lectin is a tetramer and each subunit possesses a saccharide-binding site.[2] Con A can be isolated by affinity chromatography with dextrans.[1]

Aplicação

Concanavalin A (Con A) has been used for enzyme immobilization.[3][4][5]

Used for the purification of glycoproteins containing mannose

Ações bioquímicas/fisiológicas

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca²⁺ and Mn²⁺ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Concanavalin A (Con A) is a T cell mitogen that can activate the lymphocytes.[6]
Con A can induce tumor cell death through autophagy.[7]

forma física

Suspension in 1.0 M NaCl + 0.1 M potassium phosphate, pH 6.0, with 1 mM CaCl₂, 1 mM MgCl₂ and 1 mM MnCl₂ containing preservative

Nota de análise

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca²⁺ and Mn²⁺ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H317,H334
• Declarações de precaução: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Classificações de perigo: Resp. Sens. 1 - Skin Sens. 1
• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable