C5275

Concanavalin A from Canavalia ensiformis (Jack bean)

Type IV-S, lyophilized powder, aseptically processed, BioReagent, suitable for cell culture

• Sinônimo(s): Con A
• Número CAS: 11028-71-0
• Número CE: 234-258-2
• Número MDL: MFCD00071069
• Código UNSPSC: 23151817
• NACRES: NA.32

Propriedades

• esterilidade: aseptically processed
• Nível de qualidade: 200
• tipo: Type IV-S
• linha de produto: BioReagent
• Formulário: lyophilized powder
• técnica(s): cell culture | mammalian: suitable
• Impurezas: salt (Contains buffer salts and NaCl)
• solubilidade: PBS: 5 mg/mL, slightly hazy; H₂O: soluble, slightly hazy
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Concanavalin A is a protein containing only β-sheets in its structure, and belongs to the legume lectins family.

Aplicação

Concanavalin A from Canavalia ensiformis (Jack bean) has been used:

• in in vitro cell proliferation assay and cell cycle assessment
• in lymphocyte proliferation
• in splenocyte culturing

Ações bioquímicas/fisiológicas

Concanavalin A is known to stimulate acute hepatic inflammation and acts as a T‐cell mitogen. It has inhibitory effect on hepatocellular carcinoma by inducing autophagy.

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca²⁺ and Mn²⁺ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Nota de análise

A lymphocyte transformation assay is used to determine suitability for cell culture applications. The mitogenic activity of this product is assessed by bromodeoxyuridine incorporation and is found to have peak incorporation at ≤75 ug lectin/ml of medium.
Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca²⁺ and Mn²⁺ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H317,H334,H361
• Declarações de precaução: P201 - P202 - P280 - P302 + P352 - P304 + P340 + P312 - P308 + P313
• Classificações de perigo: Repr. 2 - Resp. Sens. 1 - Skin Sens. 1
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: dust mask type N95 (US), Eyeshields, Faceshields, Gloves