C2010

Concanavalin A from Canavalia ensiformis (Jack bean)

Type IV, lyophilized powder

• Sinônimo(s): Con A
• Número CAS: 11028-71-0
• Número CE: 234-258-2
• Número MDL: MFCD00071069
• Código UNSPSC: 23151817
• NACRES: NA.32

Propriedades

• tipo: Type IV
• forma: lyophilized powder
• potência: <64 μg per mL agglutination activity
• Impurezas: Carbohydrate, essentially free; salt, essentially free
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Concanavalin A (con A) is a plant lectin refined from jack beans. It is a tetrameric protein, which has four identical 28 kDa subunits. Con A has the ability to connect with the mannose residues of several glycoproteins and can stimulate lymphocytes.

Aplicação

Concanavalin A from Canavalia ensiformis (Jack bean) has been used:

• as T-lymphocyte mitogen in lymphocyte proliferation assay for correlations with organochlorine contaminants (OCs)
• for in vitro stimulation of T cell hybridomas, BW5147 cells and primary CD4⁺ T cells for T cell vaccination and for induction of hepatitis
• for the stimulation of mice splenocytes in the suppression of T-lymphocytes proliferation assay
• for the stimulation of spleen in mitogen assays

Ações bioquímicas/fisiológicas

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca²⁺ and Mn²⁺ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Qualidade

Highly purified by specific affinity chromatography

Nota de análise

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca²⁺ and Mn²⁺ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H317,H334,H361
• Declarações de precaução: P202 - P261 - P272 - P280 - P302 + P352 - P308 + P313
• Classificações de perigo: Repr. 2 - Resp. Sens. 1 - Skin Sens. 1
• Código de classe de armazenamento: 13 - Non Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: dust mask type N95 (US), Eyeshields, Faceshields, Gloves