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A6103

Sigma-Aldrich

Aprotinin

recombinant, expressed in Nicotiana (tobacco), ≥5 TIU/mg protein, powder, ≥98% (SDS-PAGE), suitable for inhibition assay

Sinônimo(s):

BPTI, Basic pancreatic trypsin inhibitor, Kallikrein-trypsin inactivator, Kunitz protease inhibitor

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About This Item

Número CE:
Código UNSPSC:
12352202
NACRES:
NA.77

product name

Aprotinin Nicotiana tobacco, >= 5TIU/mg protein, >= 98 % SDS-PAGE | 9087-70-1, recombinant, expressed in Nicotiana (tobacco), ≥5 TIU/mg protein, ≥98% (SDS-PAGE)

recombinante

expressed in Nicotiana (tobacco)

descrição

recombinant form of thenative bovine-sequence aprotinin purified from plant tissue (tobacco)

linha de produto

BioUltra

Ensaio

≥98% (SDS-PAGE)

forma

powder

atividade específica

≥5 TIU/mg protein

peso molecular

~_6.5 kDa

técnica(s)

inhibition assay: suitable

solubilidade

0.85% sodium chloride: 5 mg/mL

nº de adesão UniProt

temperatura de armazenamento

2-8°C

Informações sobre genes

cow ... PTI(404172)

Definição da unidade

One trypsin inhibitor unit (TIU) will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmole of N-α-benzoyl-DL-arginine p-nitroanilide (BAPNA) per min at pH 7.8 at 25 °C.

Nota de preparo

Contains no animal-derived components or impurities, and is manufactured by transient expression of the aprotinin message in RNA (+)-strand tobacco mosaic virus vectors propagated in non-transgenic Nicotiana plants. This is a recombinant form of the native, bovine-sequence aprotinin, which is traditionally isolated from bovine lung by methods involving fractional precipitation, gel filtration, and ion exchange chromatography. Unlike animal-derived aprotinin, this product is isolated and purified from plant tissue by proprietary methods.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


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Artigos

While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

Protocolos

Objective: To standardize a procedure for the enzymatic assay of Aprotinin.

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