There are no protocols for using Toluidine Blue O to stain MMA sections.
198161
Toluidine Blue O
certified by the BSC
Sinônimo(s):
Basic Blue 17, Blutene chloride, Methylene Blue T50 or T extra, Tolonium chloride
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5 G
R$ 910,00
25 G
R$ 3.485,00
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5 G
R$ 910,00
25 G
R$ 3.485,00
About This Item
Fórmula empírica (Notação de Hill):
C15H16ClN3S
Número CAS:
Peso molecular:
305.83
Número do índice de cores:
52040
Número CE:
Número MDL:
Código UNSPSC:
12171500
ID de substância PubChem:
NACRES:
NA.47
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Agency
certified by the BSC
Nível de qualidade
Formulário
powder
composição
Dye content, 80%
cor
dark green
λmax
626 nm
ε (coeficiente de extinção)
≥13000 at 241-247 nm in H2O
≥28000 at 287-293 nm in H2O
≥40000 at 625-635 nm in H2O
aplicação(ões)
diagnostic assay manufacturing
hematology
histology
temperatura de armazenamento
room temp
cadeia de caracteres SMILES
[Cl-].Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C
InChI
1S/C15H15N3S.ClH/c1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;/h4-8,16H,1-3H3;1H
chave InChI
GEDVVYWLPUPJJZ-UHFFFAOYSA-N
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Descrição geral
A metachromatic dye suitable for a wide variety of histological staining procedures.
Aplicação
Ações bioquímicas/fisiológicas
Toluidine Blue O (TBO) is a thiazine dye of the quinone-imine family and is cationic in nature. It has been employed in polychromatic staining of paraffin embedded plant cell walls.[3] TBO can be removed from its aqueous solution by using an effective adsorbent, clinoptilolite.[4] TBO and fixed output laboratory laser light source are used together to destroy variety of oral bacteria associated with dental caries.[5] This metachromatic nuclear stain is often used in place of thionin or methylene blue. It also serves as a component of a tribasic stain along with Malachite Greenand Basic Fuchsin.[6]
Adequação
Certified for use as a metachromatic stain for mast cells and cartilage using the Churukian-Schenk method and the method of Nocht on paraffin sections.
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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Could you provide the protocol for using Toluidine Blue for MMA sections?
1 answer-
Helpful?
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Is there a recommendation for dissolving Toluidine Blue (198161-5g lot MKCK0257) at 1 mg/ml in water?
1 answer-
This product is released based on the testing performed by the Biological Stain Commission. There is no specific solubility specification for this product. At the BSC, the product is dissolved at 50 mg in 250 ml of water for one test, and 100 mg of the dye is dissolved in 150 ml of water for the second test, followed by the addition of additional alcohol. When used to stain frozen sections, a 0.5% solution is typically prepared in 20% alcohol. Some procedures call for dissolving at 10 mg/ml, but this amount of dye cannot always be dissolved. Once dissolved, the solution should be relatively stable.This product does not appear to be hygroscopic, and its solubility can vary somewhat from lot to lot.
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