LEU-RO

Leupeptina

powder, from synthetic, =96.5%

• Sinônimo(s): Acetil-Leu-Leu-Arg-al, N-Acetil-L-leucil-L-leucil-L-argininal
• Fórmula linear: C₂₀H₃₈N₆O₄ · 1/2H₂SO₄
• Número CAS: 103476-89-7
• Peso molecular: 475.59
• Número MDL: MFCD00037012
• Código UNSPSC: 12352202
• ID de substância PubChem: 329817679

Propriedades

• fonte biológica: synthetic
• Nível de qualidade: 100
• descrição: Ac-Leu-Leu-argininal x 1/2 H₂SO₄, synthetic
• Ensaio: 96.5%
• Formulário: powder
• peso molecular: M_r 475.6
• embalagem: pkg of 100 mg (11529048001); pkg of 25 mg (11017128001); pkg of 5 mg (11017101001); pkg of 50 mg (11034626001)
• fabricante/nome comercial: Roche
• solubilidade: H₂O: 50 mg/mL
• Condições de expedição: wet ice
• temperatura de armazenamento: 2-8°C
• cadeia de caracteres SMILES: OS(O)(=O)=O.CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C=O.CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C=O
• InChI: 1S/2C20H38N6O4.H2O4S/c2*1-12(2)9-16(24-14(5)28)19(30)26-17(10-13(3)4)18(29)25-15(11-27)7-6-8-23-20(21)22;1-5(2,3)4/h2*11-13,15-17H,6-10H2,1-5H3,(H,24,28)(H,25,29)(H,26,30)(H4,21,22,23);(H2,1,2,3,4)/t2*15-,16-,17-;/m00./s1
• chave InChI: CIPMKIHUGVGQTG-VFFZMTJFSA-N

Descrição

Especificidade

Leupeptin inhibits serine and thiol proteases such as trypsin, plasmin, proteinase K, kallikrein, papain, thrombin, and cathepsin A and B.
Not affected are α-, β-, γ- and δ- chymotrypsin, pepsin, cathepsin D, elastase, renin, and thermolysin.

Aplicação

Leupeptin as well as other protease inhibitors like antipain, chymostatin, pepstatin, and phosphoramidon are useful for the protection of proteins during their isolation from tissues or membranes. Leupeptin can be removed from the reaction by dialysis.
Note: To check other protease inhibitors, try our Protease Inhibitor Set including Antipain Dihydrochloride, Aprotinin, Bestatin, Chymostatin, E-64, EDTA-Na₂, Leupeptin, Pefabloc SC, Pepstatin, and Phosphoramidon.

Ações bioquímicas/fisiológicas

Inibidor de proteases de cisteína e serina. Inibe plasmina, tripsina, papaína, calpaína e catepsina B. Não inibe pepsina, catepsinas A e D, trombina nem α-quimotripsina. Concentração efetiva 10-100 μM. Foram realizados diversos estudos que usaram a leupeptina para proteger contra perda auditiva causada pela superestimulação acústica ou pelo antibiótico ototóxico gentamicina. (Acredita-se que a perda de células ciliadas cocleares seja mediada pela calpaína.)

Inibidor de proteases de cisteína e serina. Inibe plasmina, tripsina, papaína, calpaína e catepsina B. Não inibe pepsina, catepsinas A e D, trombina nem α-quimotripsina. Concentração efetiva 10-100 μM.

Características e benefícios

Contents
Synthetic, white powder

Qualidade

Function test: Performance controlled with trypsin.

Nota de preparo

Working concentration: 0.5 to 5 μg/ml (1 to 20 μM)

Comparison of working concentrations of pefabloc with leupeptin and PMSF is in files.

Working solution: Solvent is recommended in distilled water.
Highly soluble in water (1 mg/ml), methanol, ethanol, acetic acid, dimethyl formamide and dimethyl sulfoxide.
Poorly soluble in acetone, chloroform, ethyl ether and n-hexane.

Storage conditions (working solution): -15 to -25 °C
In aqueous solution leupeptin is stable for 1 month at 2 to 8 °C or for at least 6 months at - 15 to -25 °C, stored under nitrogen. For best results, freeze the dissolved inhibitor in aliquots and avoid repeated thawing.

Reconstituição

Highly soluble in water (1 mg/ml). Stable for at least one week at 2 to 8 °C and 6 months frozen in aliquots at -15 to -25 °C.

Nota de análise

Leupeptin gives multiple peaks on HPLC due to equilibria among three forms in solution. Purity determined using three main peaks. Majority of contaminating peptide is racemized leupeptin.

Outras notas

For life science research only. Not for use in diagnostic procedures.

Informações de segurança

• Pictogramas: GHS07
• Palavra indicadora: Warning
• Frases de perigo: H302 + H312 + H332
• Declarações de precaução: P261 - P264 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P501
• Classificações de perigo: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable