PMSF-RO
Roche
Fluoreto de fenilmetanosulfonil
Phenylmethylsulfonyl fluoride
Sinônimo(s):
αFluoreto de α-toluenosulfonil, Fluoreto de benzilsulfonil, Fluoreto de fenilmetilsulfonil, PMSF
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About This Item
Fórmula empírica (Notação de Hill):
C7H7FO2S
Número CAS:
Peso molecular:
174.19
Beilstein:
2088311
Número MDL:
Código UNSPSC:
12352202
ID de substância PubChem:
Produtos recomendados
Nível de qualidade
Ensaio
98% (TLC)
forma
solid
peso molecular
174.2
embalagem
pkg of 10 g (10837091001)
pkg of 25 g (11359061001)
fabricante/nome comercial
Roche
concentração
17-170 μg/mL
técnica(s)
western blot: suitable
pKa
7.0
pf
92-94 °C
solubilidade
>10 mg/mL
Condições de expedição
ambient
temperatura de armazenamento
20-25°C
cadeia de caracteres SMILES
FS(=O)(=O)Cc1ccccc1
InChI
1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2
chave InChI
YBYRMVIVWMBXKQ-UHFFFAOYSA-N
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Descrição geral
Soluble to >10 mg/ml in isopropanol, ethanol, methanol, or 1,2-propanediol. Unstable in aqueous solution. In 100% isopropanol, stable for at least 9 months at +25°C.
Especificidade
Inhibits serine proteases such as chymotrypsin, trypsin, and thrombin, and the cysteine protease papain (reversible by DTT treatment). Does not inhibit metalloproteases, most cysteine proteases, or aspartic proteases.
Aplicação
PMSF (C7H7O2SF) inhibits serine proteases such as chymotrypsin, trypsin, thrombin, and the cysteine protease papain (reversible by DTT treatment). It does not inhibit metalloproteases, most cysteine proteases, or aspartic proteases.
PMSF (phenylmethylsulfonyl fluoride) has been used for the preparation of protein extracts from tissues and cells prior to Western blotting.
PMSF (phenylmethylsulfonyl fluoride) has been used for the preparation of protein extracts from tissues and cells prior to Western blotting.
Ações bioquímicas/fisiológicas
PMSF (phenylmethylsulfonyl fluoride) functions as a non-specific inhibitor of multiple proteases and other enzymes, such as palmityl coenzyme A deacylase, arylsulfatase A, acetylcholinesterase, trypsin and chymotrypsin. It is an irreversible inhibitor, and sulfonylates hydroxyl groups of the serine residues present in the active site of enzymes.
Qualidade
Purity: Chromatographically homogeneous
Nota de preparo
Activator: PK requires Mg2+ (or Mn2+, CO2+) and K+ (or NH4+, Rb+) for full activity.
Working concentration: 17 to 170 μg/ml (0.1 to 1 mM)
Working solution: Due to the short half-life of PMSF, fresh solution must be added at every purification step. Under certain conditions, such as alkaline pH values, PMSF may have a half-life of only 35 minutes. High-salt conditions (3 M), often used to precipitate proteins, may also reverse the inhibition of PMSF. Pefabloc SC is recommended as a non-toxic, water-soluble, and water-stable alternative.
Storage conditions (working solution): 15 to 25 °C
Stock solutions of PMSF in 100% isopropanol are stable for at least 9 months at 15 to 25 °C.
Working concentration: 17 to 170 μg/ml (0.1 to 1 mM)
Working solution: Due to the short half-life of PMSF, fresh solution must be added at every purification step. Under certain conditions, such as alkaline pH values, PMSF may have a half-life of only 35 minutes. High-salt conditions (3 M), often used to precipitate proteins, may also reverse the inhibition of PMSF. Pefabloc SC is recommended as a non-toxic, water-soluble, and water-stable alternative.
Storage conditions (working solution): 15 to 25 °C
Stock solutions of PMSF in 100% isopropanol are stable for at least 9 months at 15 to 25 °C.
Reconstituição
Soluble to > 10 mg/ml in isopropanol, ethanol, methanol, or 1,2-propanediol. Unstable in aqueous solution. In 100% isopropanol, stable for at least 9 months at 15 to 25 °C.
Outras notas
Due to a short half-life, fresh solution must be added at every purification step. Under certain conditions, such as alkaline pH values, PMSF may only have a half-life of 35 min. High-salt conditions (3 M), often used to precipitate proteins, may also reverse the inhibition of PMSF. Pefabloc SC is recommended as a non-toxic, water-soluble, and water-stable alternative.
For life science research only. Not for use in diagnostic procedures.
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