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AP1064

Sigma-Aldrich

PhosphoDetect Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb

liquid, Calbiochem®

Sinônimo(s):

Anti-Pyruvate Dehydogenase pSer³⁰⁰ Rabbit pAb

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41
Preço e disponibilidade não estão disponíveis no momento.

fonte biológica

rabbit

Nível de qualidade

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

Formulário

liquid

contém

≤0.1% sodium azide as preservative

reatividade de espécies

human

fabricante/nome comercial

Calbiochem®

condição de armazenamento

OK to freeze
avoid repeated freeze/thaw cycles

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

phosphorylation (pSer300)

Descrição geral

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser300.
Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser300 in HEK293 cells.
This PhosphoDetect Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry for the detection of PDH-E1α (pSer³⁰⁰).

Imunogênio

Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser³⁰⁰ phosphorylation site of human PDH-E1α

Aplicação



Immunoblotting (0.5 μg/ml)
Immunocytochemistry (1 μg/ml)

Embalagem

Please refer to vial label for lot-specific concentration.

Advertência

Toxicity: Standard Handling (A)

forma física

In 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4.

Reconstituição

Following initial thaw, aliquot and freeze (-20°C).

Nota de análise

Negative Control
HEK293 cells treated with dichloroacetate
Positive Control
HEK293 cells

Outras notas

Due to a high degree of sequence identity, this antibody is also expected to cross-react with mouse, rat, bovine, Zebra fish, and Xenopus phospho-PDH-E1α Antibody should be titrated for optimal results in individual systems.
Rardin M.J., et. al. 2009. Anal. Biochem.2, 157.
Seifert, F., et al. 2007. Biochemistry 21, 6277.
Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
Patel, M.S. and Korotchkina, L.G. 2006 Biochem. Soc. Trans.34, 217.
Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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