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268298

Sigma-Aldrich

DAPI, Dihydrochloride

Cell-permeable DNA-binding dye.

Sinônimo(s):

DAPI, Dihydrochloride, 4ʹ,6-Diamidino-2-phenylindole, 2HCl

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About This Item

Fórmula empírica (Notação de Hill):
C16H15N5 · 2HCl
Número CAS:
28718-90-3
Peso molecular:
350.25
Número MDL:
MFCD00012681
Código UNSPSC:
12352200
NACRES:
NA.54

Nível de qualidade

100

descrição

RTECS - NL5995050

Ensaio

≥90% (HPLC)

Formulário

solid

fabricante/nome comercial

Calbiochem®

condição de armazenamento

OK to freeze
desiccated (hygroscopic)
protect from light

cor

yellow

solubilidade

water: 5 mg/mL
ethanol: soluble
methanol: soluble

fluorescência

λex ~359 nm
λem ~461 nm

Condições de expedição

ambient

temperatura de armazenamento

−20°C

cadeia de caracteres SMILES

[Cl-].[Cl-].[nH]1c2c(cc1c3ccc(cc3)C(=N)N)ccc(c2)C(=N)N.[H+].[H+]

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

chave InChI

FPNZBYLXNYPRLR-UHFFFAOYSA-N

Descrição geral

Cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. Useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. Can also be used to detect mycoplasma. A reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Also available as a 5 mg/ml mM solution in H2O (Cat. No. 508741).
Fluorescent dye specific for DNA. Binds preferentially to DNA rich in adenine and thymidine (excitation max. = 359 nm, emission max. = 461 nm). Useful for microscopic analysis of nuclei and nuclear DNA in apoptotic cells. Can also be used to detect mycoplasma. Reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Cells are typically stained at 1 µg/ml.

Ações bioquímicas/fisiológicas

Cell permeable: no
Primary Target
Binds preferentially to DNA rich in adenine and thymine
Product does not compete with ATP.
Reversible: yes

Advertência

Toxicity: Standard Handling (A)

Reconstituição

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 weeks at 4°C or for up to 6 months at -20°C.

Outras notas

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogramas

Exclamation mark
GHS07

Palavra indicadora

Warning

Frases de perigo

H315,H317,H335

Classificações de perigo

Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3

Órgãos-alvo

Respiratory system

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

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