Propidium iodide is soluble in water at a concentration of 1 mg/mL.
P4170
Iodeto de propídio
≥94.0% (HPLC)
Sinônimo(s):
Di-iodeto de 3,8-diamino-5-[3-(dietilmetilamônio)propil]-6-fenilfenantridínio
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10 MG
R$ 376,00
25 MG
R$ 700,00
100 MG
R$ 1.853,00
250 MG
R$ 4.078,00
500 MG
R$ 7.413,00
1 G
R$ 15.444,00
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Alterar visualização
10 MG
R$ 376,00
25 MG
R$ 700,00
100 MG
R$ 1.853,00
250 MG
R$ 4.078,00
500 MG
R$ 7.413,00
1 G
R$ 15.444,00
About This Item
Fórmula empírica (Notação de Hill):
C27H34I2N4
Número CAS:
Peso molecular:
668.39
Beilstein:
3843838
Número CE:
Número MDL:
Código UNSPSC:
41106305
ID de substância PubChem:
NACRES:
NA.77
Produtos recomendados
Nível de qualidade
Ensaio
≥94.0% (HPLC)
Formulário
powder
pf
220-225 °C (dec.) (lit.)
temperatura de armazenamento
2-8°C
cadeia de caracteres SMILES
[I-].[I-].CC[N+](C)(CC)CCC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14
InChI
1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
chave InChI
XJMOSONTPMZWPB-UHFFFAOYSA-M
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Descrição geral
O iodeto de propídio (PI) é um corante fluorescente quepode ser utilizado para a coloração de ácidos nucleicos.[1] Ele é impermeável em células viáveisou intactas. Pode penetrar facilmente em células mortas, moribundas ou com comprometimento damembrana.[1][2] O PI tem a capacidade de se ligar facilmente ao DNA.[3]
Aplicação
Características e benefícios
Este composto é um produto em destaque na pesquisa sobre apoptose. Clique aqui para saber mais sobre produtos em destaque para apoptose. Saiba mais sobre moléculas pequenas bioativas para outras áreas de pesquisa no site sigma.com/discover-bsm.
Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Muta. 2
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
dust mask type N95 (US), Eyeshields, Gloves
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Methods in molecular biology (Clifton, N.J.), 2279, 213-223 (2021-03-09)
Annexin V and propidium iodide staining is widely used for determining the cellular death through apoptosis. In the presence of Ca2+ ions, annexin V has a strong binding affinity for phosphatidylserine, a membrane phospholipid that during apoptosis is translocated from
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It has been reported that calpain/caspase-mediated apoptosis induced by endoplasmic reticulum stress (ERS) in hepatic stellate cells (HSCs) by previous studies. At present, the activation of HSC is an important cause of liver fibrosis, and the induction of HSC apoptosis
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Precise glycosylation plays a crucial and distinctive role in thymic T cell development. The core fucosylation is dramatically up-regulated at the transition from CD4-CD8- (DN) to CD4+CD8+ (DP) in the thymic development. Ablation of core fucosylation in T cells did
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Artigos
Cell cycle phases (G1, S, G2, M) regulate cell growth, DNA replication, and division in proliferating cells.
Apoptosis regulation involves multiple pathways and molecules for cellular homeostasis.
Conteúdo relacionado
Nancy-520 for DNA Detection and Quantitation
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What is the procedure for dissolving this product?
1 answer-
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Is it necessary to carry out an RNase treatment before staining with propidium iodide in my immunofluorescence assay on insect tissue fixed in 4% paraformaldehyde? Additionally, is there the complete staining protocol for propidium iodide in immunofluorescence using a confocal microscope?
1 answer-
The product has not been validated for immunofluorescence assay in our laboratory to establish a reference protocol. In cases where propidium iodide binds to both DNA and RNA, the use of RNase is common to differentiate DNA staining from undesired RNA staining. For an application protocol, one may refer to a citation of this product in an immunofluorescence assay that utilizes RNase to visualize DNA-specific staining. The citation is as follows: J Cancer. 2020 Mar 5;11(11):3274-3287. The article discusses the dual role of BI 2536, a small-molecule inhibitor targeting PLK1, in inducing apoptosis and attenuating autophagy in neuroblastoma cells. The PMID for the article is 32231733.
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Is this reagent suited for viability assays at FACS?
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While this product is not tested for any specific application, propidium iodide is commonly used in cell-based assays such as FACS. Please see the link below to review publications detailing the use of this item in flow cytometry:
https://scholar.google.com/scholar?hl=en&as_sdt=0%2C22&q=%22p4170%22+flow+cytometry&btnG=Helpful?
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What is the method to dissolve this product?
1 answer-
Propidium iodide can be dissolved in water at a concentration of 1 mg/mL. Please see the sample Certificate of Analysis for solubility information: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/P4170-BULKWXBF1544V.pdf
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