The use of this product for hydroponic cultures has not been studied, so suitability cannot be confirmed. The 1L container contains 1.6 grams of powder, which is enough to make 1 liter of solution.
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H2395
Hoaglands Basalsalzmischung Nr. 2
powder, suitable for plant cell culture
Synonym(e):
Hoagland solution 2
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
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1 L
€ 28,30
10 L
€ 95,70
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1 L
€ 28,30
10 L
€ 95,70
About This Item
UNSPSC-Code:
12352207
NACRES:
NA.72
Empfohlene Produkte
Form
powder
Qualitätsniveau
Methode(n)
cell culture | plant: suitable
Anwendung(en)
agriculture
Lagertemp.
2-8°C
Anwendung
Hoaglands Basalsalzmischung Nr. 2 hat folgende Anwendungen:
- Herstellung von Hoagland-Lösung zur Behandlung von Wurzelspitzen[1]
- Züchtung von Maissamen (Zea mays L.)[2]
- Züchtung von Reiskeimlingen[3] Hydroloponische Behandlung von M. sinensis (cv. Kosung)-Keimlingen[4]
Hoaglands Basalsalzmischung Nr. 2 ist ein klassisches Pflanzenmedium. Hoagland-Lösung findet breite Anwendung mit einer großen Vielzahl von Pflanzenspezies.
Alternative Formulierung
Menge
Formulierung enthält 1,6 Gramm Pulver pro Liter Nährmedium.
Ähnliches Produkt
Produkt-Nr.
Beschreibung
Preisangaben
Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Acute Tox. 4 Oral - Eye Dam. 1 - Ox. Sol. 3
Lagerklassenschlüssel
5.1B - Oxidizing hazardous materials
WGK
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Zulassungslistungen
Zulassungslistungen werden hauptsächlich für chemische Produkte erstellt. Für nicht-chemische Produkte können hier nur begrenzte Angaben gemacht werden. Kein Eintrag bedeutet, dass keine der Komponenten gelistet ist. Es liegt in der Verantwortung des Benutzers, die sichere und legale Verwendung des Produkts zu gewährleisten.
EU REACH SVHC Candidate List
CAS No.
EU REACH Annex XVII (Restriction List)
CAS No.
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Analysenzertifikate (COA)
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The water-culture method for growing plants without soil.
Hoagland, D.R., and Arnon, D.I.
Univ. Calif. Coll. Agric. Exp. Sta. Circ., 347-353 (1938)
Dongling Mou et al.
Ecotoxicology and environmental safety, 74(4), 776-786 (2010-11-16)
The cuttings of grape (Vitis vinifera Linn.) were exposed to Hoagland's solution containing five different manganese (Mn) concentrations to investigate Mn toxicity and the possible detoxifying responses. Three genotypes (i.e. cultivars Combiner, Jingshou and Shuijing) were used in present study.
Yunying Fang et al.
Global change biology, 24(7), 2775-2790 (2018-04-01)
Agronomic practices such as crop residue return and additional nutrient supply are recommended to increase soil organic carbon (SOC) in arable farmlands. However, changes in the priming effect (PE) on native SOC mineralization in response to integrated inputs of residue
Renee S Arias et al.
BMC plant biology, 18(1), 170-170 (2018-08-17)
Aflatoxin contamination in peanut seeds is still a serious problem for the industry and human health. No stable aflatoxin resistant cultivars have yet been produced, and given the narrow genetic background of cultivated peanuts, wild species became an important source
Jin Seok Yoon et al.
Plant cell reports, 38(9), 1109-1125 (2019-05-28)
BdASR4 expression was up-regulated during abiotic stress and hormone treatments. Plants over-expressing BdASR4 improved drought tolerant. BdASR4 may regulate antioxidant activities and transcript levels of stress-related and abscisic acid-responsive genes. Abiotic stress conditions negatively affect plant growth and developmental processes
Artikel
Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.
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Hello The Hoagland salt is for in vitro cell culture? I need it for hydroponic culture only. you think its suitable? and another question: 1 L means that its the weight of bottle? or it is just enough for making 1 liter soution of Hoagland?
1 answer-
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Good day Do you have Hoagland's solution that supply all elements except Nitrogen?
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Currently, this product is the only Hoagland's basal salt mixture available. This is a powdered medium that is prepared at 1.6 grams per liter. The formulation includes Calcium Nitrate. Please see the link below to review the formulation of this and other plant basal salt mixtures and media:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/137/040/h2395for.pdfHelpful?
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Can the Hoagland's solution be autoclaved?
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Yes, this product in solution can be autoclaved for sterilization. Please find the preparation instructions below.
This powder is extremely hygroscopic and must be protected from atmospheric moisture. Do not open the container until its contents are allowed to warm to room temperature. If possible the entire contents of the package should be used immediately after opening.
1.6 g of powder is required to prepare 1 L of medium.
Preparing this product in a concentrated form is not recommended as some salt complexes may precipitate. Supplements may be added prior to sterilization or added aseptically to a sterile medium. Certain supplements (i.e. heat labile) may require filter sterilization and may affect the shelf life of the medium.
The basic steps for preparing culture medium are the following:
1. Using a container twice the size of the desired final volume, measure out approximately 90% of the final required volume of tissue culture grade water (e.g. Sigma Product No. W-3500). Example: 900 ml for a final volume of 1000 ml.
2. While stirring, add the powdered product.
3. Rinse the original container with a small volume of tissue culture-grade water to remove traces of the powder. Add to the solution in Step 2.
4. Add any desired supplements (e.g. vitamins, gelling agent, auxin, cytokinin, etc.).
5. While stirring, adjust to the desired pH (e.g. 5.7 +/- 0.1) using KOH, NaOH, or HCl.
6. Add additional tissue culture-grade water to bring the medium to the final volume.
7. If a gelling agent is used, heat the solution to approximately 100°C while stirring.
8. Dispense the medium into culture vessels before or after autoclaving according to your application. Add heat labile constituents after autoclaving.
9. Sterilize the medium in a validated autoclave at 1 kg/cm2 (15 psi). The medium should attain a temperature of 121°C for at least 15 min.All media preparations should be stored at 0-5°C. Store dry powder in a desiccator. Deterioration of powdered medium may be recognized by the following: 1) color change; 2) granulation, clumping, or particulate matter throughout the powder; 3) pH change; and 4) inability to promote growth when properly used.
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Why do we use ferric tartarte in this medium. Please specify the role of ferric tartarte. Can ferric tartarte be replaced with ferric sulphate or ferric citrate?
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Ferric tartrate is used in the original Hoagland's classical medium formulation developed in the 1930s. This product is a duplication of that formulation. This compound serves as the source of iron in this specific formulation. The most common source of iron utilized in plant basal salt mixtures is Ferrous Sulfate Heptahydrate. Ferric Citrate is less commonly used, however it is present in several Lindemann Orchid salt mixtures. Other iron sources may be suitable, but these have not been evaluated in published classical media formulations.
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Hello, I am in need of some Hoagland solution for my pots. I am in need of 20l of half strength Hoagland solution. Can you provide the technical details and how to use this solution ?
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The 1L and 10L package sizes are indicative of the amount of basal salt solution that can be prepared from the powder within. The 1L package contains 1.6 grams and the 10L package contains 16 grams. To prepare at half strength, simply double the volume of water to be added. The 1L package can be diluted in 2L of water and the 10L package can be diluted in 2L of water. Stirring will be required.
Note that this powder is extremely hygroscopic and must be protected from atmospheric moisture. Allow the container to reach room temperature prior to opening.
Please see the link below for additional preparation instructions:
https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/plant-tissue-culture/media-preparationHelpful?
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1.6g of powder makes 1L of solution - how many grams is in the 1L container of powder? I.e. how many litres can be made using the 1L of powder
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The 1L and 10L package sizes are indicative of the amount of basal salt solution that can be prepared from the powder within. There is a sufficient amount of powder to prepare either 1 or 10 liters in each bottle, 1.6 grams and 16 grams, respectively. Note that this powder is extremely hygroscopic and must be protected from atmospheric moisture. Allow the container to reach room temperature prior to opening.
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How many L of Hoagland Solution full strength can I prepare with 1.6 g of SKU H2395?
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The classical Hoagland's No. 2 formulation shows 1.6 G of the basal salt mixture is sufficient to prepare 1L of medium, This product is a dry powder medium preweighed to prepare the volume stated - 1L and 10L.
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is the hoagland's no. 2 basalt mixture powder 1 ltr, 1 ltr of powder or one ltr of solution or enough powder to make one liter of solution ?
1 answer-
For the SKU H2395-1L, the material is formulated to contain 1.6 grams of powder which is reconstituted to a final volume of 1L of medium.
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