F9015

Fluorescamin

≥98% (TLC), powder, used for detection of primary amines

• Synonym(e): 4-Phenylspiro-[furan-2(3H),1-phthalan]-3,3′-dion
• Empirische Formel (Hill-System): C₁₇H₁₀O₄
• CAS-Nummer: 38183-12-9
• Molekulargewicht: 278.26
• UNSPSC Code: 12352204
• NACRES: NA.32
• PubChem Substance ID: 24894984
• EC Number: 253-814-5
• Beilstein/REAXYS Number: 921143
• MDL number: MFCD00005928

Eigenschaften

• assay: ≥98% (TLC)
• form: powder
• color: off-white to yellow
• mp: 153-157 °C (lit.)
• solubility: acetone: 50 mg/mL
• storage temp.: room temp
• SMILES string: O=C1OC2(OC=C(C2=O)c3ccccc3)c4ccccc14
• InChI: 1S/C17H10O4/c18-15-13(11-6-2-1-3-7-11)10-20-17(15)14-9-5-4-8-12(14)16(19)21-17/h1-10H
• InChI key: ZFKJVJIDPQDDFY-UHFFFAOYSA-N

Beschreibung

General description

Fluorescamine, a heterocyclic dione, reacts with primary amines, amino acids, peptides, and protein to form a fluorescent product. Excess reagent and its degradation products are non-fluorescent. The fluorescence of a solution containing protein plus fluorescamine is equivalent to the quantity of free amine groups present. This is the basic principle of a fluorescent protein assay. Fluorescamine is used as a derivatizing agent for the identification of various compounds used in the pharmaceutical industry, such as oseltamavir, penicillamine, sulfonamide residues, lisinopril, methotrexate, vigabatrin, and gabapentin.

Application

Ein nicht fluoreszierendes Reagenz, das unter milden Bedingungen schnell mit primären Aminen in Aminosäuren und Peptiden reagiert und stabile, stark fluoreszierende Verbindungen bildet. Geringer Hintergrund aufgrund der Hydrolyse. Für die fluorometrische Bestimmung von Aminosäuren, Proteinen und proteolytischen Enzymen geeignet. Blockiert effektiv neu gebildete Aminotermini in Proteinsequenzanalysen.

Fluorescamine has been used as a stain to localize granular proteins on the surface of foxtail millet starch as well as protein in foxtail millet flour through confocal scanning laser microscopy (CSLM). It has also been used for photometric determination of para-aminobenzoic acid.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)