The composition of the media can be found at the following link:
https://www.sigmaaldrich.com/technical-documents/protocol/cell-culture-and-cell-culture-analysis/plant-tissue-culture/phycology-media#table
B5282
Bold Modified Basal Freshwater Nutrient Solution
50 ×, liquid, suitable for plant cell culture
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NACRES:
NA.72
UNSPSC Code:
10171502
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Unterstützung erhaltensterility
sterile-filtered
Quality Level
form
liquid
concentration
50 ×
technique(s)
cell culture | plant: suitable
application(s)
agriculture
storage temp.
2-8°C
1 of 4
Dieser Artikel | |||
|---|---|---|---|
| technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable |
| form liquid | form liquid | form liquid | form liquid |
| application(s) agriculture | application(s) agriculture | application(s) agriculture | application(s) agriculture |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 400 | Quality Level 400 | Quality Level 400 | Quality Level 400 |
Application
Bold Modified Basal Freshwater Nutrient Solution, 50x is a chemstock used to prepare Bold Modified Freshwater Nutrient Solution for use in phycology/algae studies.
Preparation Note
With major salts and trace elements. Modified with trace elements as requested for ASTM TOXICITY TESTS E1193 and E1295 conducted with Daphnia species.
Lagerklasse
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 2
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Sing-Lai Lim et al.
Bioresource technology, 101(19), 7314-7322 (2010-06-16)
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Eloísa Ramos-Rodríguez et al.
The Journal of experimental biology, 223(Pt 7) (2020-02-29)
Organisms with wide environmentally induced morphological plasticity and cosmopolitan distribution, e.g. the common freshwater rotifer Keratella cochlearis, are ideal models to study the evolution of plastic polymorphisms and the capacity of zooplankton to adapt to local selection conditions. We investigated
Osamu Nishimura et al.
PloS one, 10(11), e0143525-e0143525 (2015-11-21)
We established a laboratory clonal strain of freshwater planarian (Dugesia japonica) that was derived from a single individual and that continued to undergo autotomous asexual reproduction for more than 20 years, and we performed large-scale genome sequencing and transcriptome analysis
Karina M Michetti et al.
Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al, 30(3), 491-496 (2007-03-23)
Nonspecific acid phosphatases are a group of enzymes whose activity increases the availability of exogenous and endogenous orthophosphate either through extra- or intracellular hydrolysis of phosphate compounds. Our study demonstrates the activity of acid phosphatases in the filamentous freshwater alga
Jakub Nedbal et al.
HardwareX, 8, e00143-e00143 (2021-01-15)
A bottom-illuminated orbital shaker designed for the cultivation of microalgae suspensions is described in this open-source hardware report. The instrument agitates and illuminates microalgae suspensions grown inside flasks. It was optimized for low production cost, simplicity, low power consumption, design
Protokolle
Phycology media can be prepared from concentrated solutions or from powdered salt mixtures. The concentrated solutions are complete, including vitamins, and should be stored frozen.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| B5282-500ML | 04061833433713 |
| B5282-500MLSTAB | 04065272739757 |
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¿Podrían informarme de la composición de B5282, Bold Modified Basal Freshwater Nutrient Solution?
1 answer-
Helpful?
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Can this medium be combined with agar to make a solid medium?
1 answer-
While not tested as such, a gelling agent may be added to this solution to form a solid medium. As this is a prepared solution, the agar would be added directly to the medium.
Please see the link below, step 7, to review the general phycology media preparation protocol: https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/plant-tissue-culture/phycology-media
Helpful?
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Is this suitable for freshwater diatom culture?
1 answer-
This product is tested for culture using green algae that are not specifically designated as diatomaceous. This application may need to be validated by the end user. The formulation is prepared according to the the reference below:
Nichols, H. W. Growth Media-Freshwater in: Stein, J.r. (ed) Phycological Methods, Cambridge Univ. Press, 7-23, 1973.Helpful?
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