While the specifications for this probe do not include assaying for specific applications, there are many publications showing its use in flow cytometry applications:
https://www.sciencedirect.com/science/article/pii/S0022030215000909
https://iubmb.onlinelibrary.wiley.com/doi/pdf/10.1002/iub.1895
https://www.tandfonline.com/doi/pdf/10.1080/21655979.2021.1994737
https://journal.waocp.org/article_91422_6ccd2a19eb9159073edeb8f9ce0be660.pdf
https://pmc.ncbi.nlm.nih.gov/articles/PMC7842666/
Alle Fotos(2)
Wichtige Dokumente
D6883
2′,7′-Dichlorfluorescein-Diacetat
≥97% (HPLC), powder, cell-permeable non-fluorescent probe
Synonym(e):
DCFH-DA, 2′,7′-Dichlor-dihydrofluorescein-diacetat
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Größe auswählen
50 MG
€ 61,50
250 MG
€ 246,00
Alle Fotos(2)
Größe auswählen
Ansicht ändern
50 MG
€ 61,50
250 MG
€ 246,00
About This Item
Empirische Formel (Hill-System):
C24H16Cl2O7
CAS-Nummer:
Molekulargewicht:
487.29
Beilstein:
4610273
MDL-Nummer:
UNSPSC-Code:
12352200
PubChem Substanz-ID:
NACRES:
NA.77
Empfohlene Produkte
Produktbezeichnung
2′,7′-Dichlorfluorescein-Diacetat, ≥97%
Qualitätsniveau
Assay
≥97%
Form
powder
Lagertemp.
−20°C
SMILES String
CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C(c2cc1Cl)c4ccccc4C(O)=O
InChI
1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)
InChIKey
PXEZTIWVRVSYOK-UHFFFAOYSA-N
Suchen Sie nach ähnlichen Produkten? Aufrufen Leitfaden zum Produktvergleich
Allgemeine Beschreibung
2′,7′-Dichlordihydrofluorescein-Diacetat (DCFH-DA) ist ein oxidationsempfindlicher fluoreszierender Farbstoff, der zum Nachweis der intrazellulären Konzentration der reaktiven Sauerstoffspezies (ROS) verwendet wird. DCFH-DA ist eine zelldurchlässige, nicht fluoreszierende Sonde, die durch intrazelluläre Esterase zu DCFH hydrolysiert werden kann, die bei Oxidation durch die intrazellulären ROS eine fluoreszierende DCF bildet.[1]
In Ethanol beträgt die Anregungswellenlänge 504 nm und die Emissionswellenlänge 529 nm. Die Verwendung von DMSO als Lösungsmittel wurde nicht untersucht, aber verschiedene Veröffentlichungen legen nahe, dass es geeignet ist.
Anwendung
2′,7′-Dichlorfluoreszeindiacetat eignet sich zur Verwendung in:
- der sensitiven und schnelle Quantifizierung von reaktiven Sauerstoffspezies als Reaktion auf den oxidativen Metabolismus[2][1][3]
- Mikrotiterplatten-Assays für den Nachweis von oxidativen Produkten in phagozytischen Zellen
- quantitative Multiwell-Myeloid-Differenzierungs-Assays
Anwendungsgebiete sind die empfindliche und rasche Quantifizierung von reaktiven Sauerstoffspezies in Reaktion auf oxidative Stoffwechselvorgänge, Mikroplatten-Assays für den Nachweis von Oxidationsprodukten in Fresszellen sowie quantitative Multiwell-Assays zur Differenzierung von myeloischen Zellen.
Lagerung und Haltbarkeit
Dieses Produkt sollte als Pulver bei -20 °C gelagert werden.
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
Hier finden Sie alle aktuellen Versionen:
Analysenzertifikate (COA)
Lot/Batch Number
Die passende Version wird nicht angezeigt?
Wenn Sie eine bestimmte Version benötigen, können Sie anhand der Lot- oder Chargennummer nach einem spezifischen Zertifikat suchen.
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Kunden haben sich ebenfalls angesehen
Katia Cortese et al.
Life sciences, 232, 116610-116610 (2019-06-30)
The aim of this study was the characterization of the in vitro cytotoxic properties of a recently isolated diterpene compound, 7β-acetoxy-20-hydroxy-19,20-epoxyroyleanone (compound 1), extracted from Salvia corrugata, versus human cell lines. We used as model study immortalized breast epithelial cells
Pornthip Chaichompoo et al.
Journal of clinical pathology, 72(8), 520-524 (2019-04-24)
Iron overload is a major factor contributing to the overall pathology of thalassaemia, which is primarily mediated by ineffective erythropoiesis and shorter mature red blood cell (RBC) survival. Iron accumulation in RBCs generates reactive oxygen species (ROS) that cause cellular
Yuan Ji et al.
Journal of agricultural and food chemistry, 67(32), 9060-9069 (2019-07-25)
Glutathione S-transferases (GSTs) play an active role in the development of drug resistance by numerous cancer cells, including melanoma cells, which is a major cause of chemotherapy failure. As part of our continuous effort to explore why dietary polyphenols bearing
Yeonhwa Song et al.
Journal of experimental & clinical cancer research : CR, 36(1), 38-38 (2017-03-04)
CD133-positive cells in hepatocellular carcinoma (HCC) exhibit cancer stem cell (CSC)-like properties as well as resistance to chemotherapeutic agents and ionizing radiation; however, their function remains unknown. In this paper, we identified a hitherto unknown mechanism to overcome CD133-induced resistance
Hung-Chi Tu et al.
Ecotoxicology and environmental safety, 182, 109380-109380 (2019-07-07)
Ultraviolet (UV) is an omnipresent environmental carcinogen transmitted by sunlight. Excessive UV irradiation has been correlated to an increased risk of skin cancers. UVB, the most mutagenic component among the three UV constituents, causes damage mainly through inducing DNA damage
Artikel
Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.
DISCOVER Bioactive Small Molecules for Nitric Oxide & Cell Stress Research
-
Is D6883 2´,7´-Dichlorofluorescin diacetate suitable for flow cytometry analyses?
1 answer-
Helpful?
-
-
What is the protocol for measuring ROS using D6883? Specifically, what kind of media or buffer is used with this product?
1 answer-
The specific protocol for measuring ROS using D6883 and the type of media or buffer to be used with this product are not available. However, typically, this product is added to a buffer solution shortly before assessing the fluorescent signal. For further guidance, refer to the following citations for relevant information:
1. Novel Antioxidant Tripeptide "ACQ" Can Prevent UV-Induced Cell Death and Preserve the Number of Epidermal Stem Cells
PMID: 261996772. Detection of reactive oxygen species (ROS) by the oxidant-sensing probe 2',7'-dichlorodihydrofluorescein diacetate in the cyanobacterium Anabaena variabilis PCC 7937
PMID: 2057064These publications may provide insights into the protocol for using D6883 and the type of media or buffer used with this product.
Helpful?
-
-
What concentration of DCF can I use for A549 cells?
1 answer-
This product has not been tested for use in cell culture. A search of the literature suggests working concentrations range from 5 µM to 0.1 mM. See the link below to a publication that specifically looked at A549 cells using several dyes, including DCFDA:
https://pubs.rsc.org/en/content/articlehtml/2017/ra/c7ra01025gHelpful?
-
-
What concentration of DCF can I use for THP-1 monocyte cell line?
1 answer-
The optimal concentration of 2′,7′-Dichlorofluorescin diacetate (DCFDA) for use with THP-1 monocytes will need to be determined by the end-user. See the link below to a paper that reported using DCF at 40 uM with THP-1 cells.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8961584/Helpful?
-
-
Is it possible to view this probe under a fluorescence microscope if the probe is solubilised in DMSO? What are the excitation and absorption wavelengths?
1 answer-
The excitation wavelength is 504 nm and the emission wavelength is 529 nm in ethanol. The use of DMSO as the solvent has not been determined, however, various publications suggest that it is suitable. See the link below for an example:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712457/Helpful?
-
-
Protocol please! Thank you very much!
1 answer-
It may be helpful to view the following articles as there is not a specific protocol for this product:
Helpful?
-
-
Can the product be stored in powder form and if its possible what is the storage temp?
1 answer-
This product should be stored in powdered format at -20°C.
Helpful?
-
-
What is the solution stability of Product D6883, 2´,7´-Dichlorofluorescin diacetate?
1 answer-
This product can be stored at -20°C for up to six months in solvents such as DMSO, ethanol, or DMF (N,N-dimethylformamide).
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
What is the excitation and emission wavelength for Product D6883, 2´,7´-Dichlorofluorescin diacetate?
1 answer-
The excitation wavelength is 504 nm and emission wavelength is 529 nm (in ethanol).
Helpful?
-
Active Filters
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung.
