The solution stability of this specific product has not been determined, but solutions are quite stable. Product P4864, a 1 mg/mL prepared solution, is stored refrigerated and offers a warranty of 1 year from the date of shipment.
P4170
Propidiumjodid
≥94.0% (HPLC), fluorescent dye, powder
Synonym(e):
3,8-Diamino-5-(3-diethylaminopropyl)-6-phenyl-phenanthridiniumiodid-methiodid
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10 MG
€ 63,40
25 MG
€ 105,00
100 MG
€ 314,00
250 MG
€ 392,00
500 MG
€ 714,00
1 G
€ 1.720,00
Über diesen Artikel
Empirische Formel (Hill-System):
C27H34I2N4
CAS-Nummer:
Molekulargewicht:
668.39
NACRES:
NA.77
PubChem Substance ID:
UNSPSC Code:
41106305
EC Number:
247-081-0
MDL number:
Beilstein/REAXYS Number:
3843838
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Unterstützung erhaltenProduktname
Propidiumjodid, ≥94.0% (HPLC)
InChI key
XJMOSONTPMZWPB-UHFFFAOYSA-M
InChI
1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
SMILES string
[I-].[I-].CC[N+](C)(CC)CCC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14
assay
≥94.0% (HPLC)
form
powder
mp
220-225 °C (dec.) (lit.)
storage temp.
2-8°C
Quality Level
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Verwandte Kategorien
1 of 4
Dieser Artikel | |||
|---|---|---|---|
| assay ≥94.0% (HPLC) | assay ≥94% (HPLC) | assay - | assay - |
| form powder | form powder | form solid | form liquid |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| mp 220-225 °C (dec.) (lit.) | mp 220-225 °C (dec.) (lit.) | mp - | mp - |
General description
Propidiumiodid (PI) ist ein fluoreszierender Farbstoff, derzur Färbung von Nukleinsäuren verwendet werden kann.[1] Es kann nicht in lebensfähige oderintakte Zellen eindringen. Er kann leicht in abgestorbene und sterbende Zellen sowie Zellen mit beeinträchtigter Membran eindringen.[1][2] PI bindet leicht an DNA.[3]
Application
Features and Benefits
Diese Verbindung ist ein wichtiges Produkt für die Forschung im Bereich der Apoptose. Weitere empfohlene Apoptoseprodukte finden Sie hier Weitere Informationen über bioaktive kleine Moleküle für andere Forschungsbereiche finden Sie hier: sigma.com/discover-bsm.
signalword
Warning
hcodes
Hazard Classifications
Muta. 2
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Wei Liang et al.
Molecular immunology, 112, 312-321 (2019-06-24)
Precise glycosylation plays a crucial and distinctive role in thymic T cell development. The core fucosylation is dramatically up-regulated at the transition from CD4-CD8- (DN) to CD4+CD8+ (DP) in the thymic development. Ablation of core fucosylation in T cells did
Julia Burrill et al.
Forensic science international. Genetics, 46, 102269-102269 (2020-03-11)
Forensic DNA typing from touched or handled items in routine casework is increasing as the sensitivity of detection techniques improves. Our understanding of the cellular/acellular content of touch deposits and the origins of the DNA therein is still limited. This
Robin Kumar et al.
Methods in molecular biology (Clifton, N.J.), 2279, 213-223 (2021-03-09)
Annexin V and propidium iodide staining is widely used for determining the cellular death through apoptosis. In the presence of Ca2+ ions, annexin V has a strong binding affinity for phosphatidylserine, a membrane phospholipid that during apoptosis is translocated from
Aswathy Shailaja et al.
Biofilm, 4, 100090-100090 (2022-11-18)
Filamentous fungi are ubiquitous and frequent components of biofilms. A means to visualize them and quantify their viability is essential for understanding their development and disruption. However, quantifying filamentous fungal biofilms poses challenges because, unlike yeasts and bacteria, they are
Haiying Liu et al.
Frontiers in cell and developmental biology, 9, 684857-684857 (2021-10-05)
It has been reported that calpain/caspase-mediated apoptosis induced by endoplasmic reticulum stress (ERS) in hepatic stellate cells (HSCs) by previous studies. At present, the activation of HSC is an important cause of liver fibrosis, and the induction of HSC apoptosis
Artikel
Apoptosis regulation involves multiple pathways and molecules for cellular homeostasis.
Cell cycle phases (G1, S, G2, M) regulate cell growth, DNA replication, and division in proliferating cells.
Verwandter Inhalt
Nancy-520 for DNA Detection and Quantitation
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How long can we stored PI dissolved in water?
1 answer-
Helpful?
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Is it necessary to carry out an RNase treatment before staining with propidium iodide in my immunofluorescence assay on insect tissue fixed in 4% paraformaldehyde? Additionally, is there the complete staining protocol for propidium iodide in immunofluorescence using a confocal microscope?
1 answer-
The product has not been validated for immunofluorescence assay in our laboratory to establish a reference protocol. In cases where propidium iodide binds to both DNA and RNA, the use of RNase is common to differentiate DNA staining from undesired RNA staining. For an application protocol, one may refer to a citation of this product in an immunofluorescence assay that utilizes RNase to visualize DNA-specific staining. The citation is as follows: J Cancer. 2020 Mar 5;11(11):3274-3287. The article discusses the dual role of BI 2536, a small-molecule inhibitor targeting PLK1, in inducing apoptosis and attenuating autophagy in neuroblastoma cells. The PMID for the article is 32231733.
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What is the procedure for dissolving this product?
1 answer-
Propidium iodide is soluble in water at a concentration of 1 mg/mL.
Helpful?
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Is this reagent suited for viability assays at FACS?
1 answer-
While this product is not tested for any specific application, propidium iodide is commonly used in cell-based assays such as FACS. Please see the link below to review publications detailing the use of this item in flow cytometry:
https://scholar.google.com/scholar?hl=en&as_sdt=0%2C22&q=%22p4170%22+flow+cytometry&btnG=Helpful?
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What is the method to dissolve this product?
1 answer-
Propidium iodide can be dissolved in water at a concentration of 1 mg/mL. Please see the sample Certificate of Analysis for solubility information: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/P4170-BULKWXBF1544V.pdf
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