This compound is used extensively for the staining of living cells. It has been used as a viability stain for cultured neurons and plant ways, and in measuring of esterase and cholinesterase activity. See the following references for usage: Fluorometric determination of lipase: Guilbault, G.G. and Kramer, D.N., "Fluorometric Determination of Lipase, Acylase, Alpha- and Gamma- Chymotrypsin and Inhibitors of These Enzymes", Anal. Chem. 36, 409, 1964. ; Anal. Biochem., Vol. 14, 28 (1966);Fluorescent stain for viable rosette-forming cells: J. Immun. Meth., Vol. 5,305 (1974);Fluorogenic substrate detection of viable pathogenic protozoa: Science, Vol. 227, 435 (1985);Reference Standard in antibody studies: Anal. Biochem., Vol. 9, 474 (1964);Cell viability staining: Staining Procedures, G. Clark, 4th ed., p. 93.Guilbault, G.G. and Kramer, D.N., "Fluorometric Determination of Lipase, Acylase, Alpha- and Gamma- Chymotrypsin and Inhibitors of These Enzymes, Anal. Chem. 36, 409, 1964."" "
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F7378
Fluoreszeindiacetat
Powder
Synonym(e):
Di-O-acetylfluorescein, Fluorescein-diacetat
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About This Item
Empfohlene Produkte
Produktbezeichnung
Fluoreszeindiacetat, used as cell viability stain
Qualitätsniveau
Form
powder
Methode(n)
titration: suitable
Farbe
white to faint yellow
mp (Schmelzpunkt)
200-203 °C (lit.)
Löslichkeit
acetone: 25 mg/mL
ε (Extinktionskoeffizient)
≥86000 at 488-494 nm in 0.1 M NaOH
Anwendung(en)
diagnostic assay manufacturing
hematology
histology
Lagertemp.
room temp
SMILES String
CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccccc45)c1
InChI
1S/C24H16O7/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24/h3-12H,1-2H3
InChIKey
CHADEQDQBURGHL-UHFFFAOYSA-N
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Allgemeine Beschreibung
Anwendung
Substrate
Ähnliches Produkt
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
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What are some common applications for Product F7378, Fluorescein diacetate?
1 answer-
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After Product F7378, Fluorescein diacetate, has been converted to fluorescein, what are the maximum excitation and emission wavelengths?
1 answer-
The maximum excitation wavelength is 490 nm and the maximum emission wavelength is 526 nm.
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How stable are solutions of Product F7378, Fluorescein diacetate, once prepared?
1 answer-
Based on our experience, stock solutions of fluorescein diacetate at 5 mg/mL in acetone or DMSO can be stored at -20 °C. According to one on-line source, solutions at 5 mg/mL in acetone or ethanol can be stored refrigerated.
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Is Product F7378, Fluorescein diacetate, a fluorescent compound?
1 answer-
No. Only upon hydrolysis of the esters, catalyzed by cellular esterase, the lactone ring opens yielding fluorescein. Only the fluorescein form is fluorescent.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What solubility information do you have on Product F7378, Fluorescein diacetate?
1 answer-
We have found that it is slightly soluble in water at 0.1%, and in ethanol at 0.3%. It is also soluble in dimethylformamide (DMF).
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