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W1754

Sigma-Aldrich

Wasser

PCR Reagent, suitable for PCR

Synonym(e):

H2O

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About This Item

Lineare Formel:
H2O
CAS-Nummer:
7732-18-5
Molekulargewicht:
18.02
Beilstein:
2050024
EG-Nummer:
231-791-2
MDL-Nummer:
MFCD00011332
UNSPSC-Code:
12191602
PubChem Substanz-ID:
24900779
NACRES:
NA.25

product name

Wasser, PCR Reagent

Qualität

PCR Reagent

Qualitätsniveau

200

Dampfdichte

<1 (vs air)

Dampfdruck

3 mmHg

Sterilität

sterile-filtered

Form

liquid

Verpackung

vial of 1.5 mL

Methode(n)

PCR: suitable

Brechungsindex

n20/D 1.34 (lit.)

pH-Wert

5-7

bp

100 °C (lit.)

mp (Schmelzpunkt)

0 °C (lit.)

Dichte

1.000 g/mL at 3.98 °C (lit.)

Fremdaktivität

DNase, none detected
RNase, none detected

SMILES String

O

InChI

1S/H2O/h1H2

InChIKey

XLYOFNOQVPJJNP-UHFFFAOYSA-N

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Allgemeine Beschreibung

PCR grade water is sterile-filtered. It is free from exonucleases (DNAse, RNAse) and endonuclease (NICKase) and is also free from nucleic acid contamination.

Anwendung

Water has been used:

  • as a component of the reaction mixture and as a diluting agent in microfluidic RT-qPCR
  • as a component of the reaction mixture for the amplification of products from fungal (Trametes versicolor) DNA
  • as a diluting agent and as a component of the reaction mixture for the amplification of cDNA
  • Water has been used to make up the final volume of the sample in polymerase chain reaction (PCR)

Eignung

Suitable for polymerase chain reaction (PCR)

Sonstige Hinweise

Easily compare specifications for Water products with the Water specification table.

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Francine Marciano-Cabral et al.
Applied and environmental microbiology, 69(10), 5864-5869 (2003-10-09)
The free-living amoeboflagellate Naegleria fowleri is the causative agent of primary amoebic meningoencephalitis (PAM), a rapidly fatal disease of the central nervous system. In the United States, the disease is generally acquired while swimming and diving in freshwater lakes and
J Loeffler et al.
Journal of clinical microbiology, 37(4), 1200-1202 (1999-03-13)
Successful in vitro amplification of fungal DNA in clinical specimens has been reported recently. In a collaboration among five European centers, the frequency and risk of contamination due to airborne spore inoculation or carryover contamination in fungal PCR were analyzed.
Incidence and survival of non-O157 verocytotoxigenic Escherichia coli in soil
Bolton DJ, et al.
Journal of Applied Microbiology, 111(2), 484-490 (2011)
Multiplex PCR for rapid detection of genes encoding acquired metallo-beta-lactamases.
Matthew J Ellington et al.
The Journal of antimicrobial chemotherapy, 59(2), 321-322 (2006-12-23)
N Wellinghausen et al.
Applied and environmental microbiology, 67(9), 3985-3993 (2001-08-30)
Contamination of hospital water systems with legionellae is a well-known cause of nosocomial legionellosis. We describe a new real-time LightCycler PCR assay for quantitative determination of legionellae in potable water samples. Primers that amplify both a 386-bp fragment of the
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Examination of Lentiviral Library Efficiency

Small interfering RNAs (siRNAs) are powerful tools for gene expression knockdown, widely used in molecular biology.

Protokolle

Hot Start dNTP Protocol

Hot start dNTP protocol enhances specificity in PCR by blocking DNA polymerase nucleotide incorporation during PCR.

SYBR® Green JumpStart™ Taq ReadyMix™

Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.

Long and Accurate PCR Amplification of DNA (D8045)

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.

Reverse Transcription Protocol (One-step Probe Detection)

Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.

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