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W4502

Wasser

suitable for electrophoresis, PCR

Water

Synonym(e):

DNasefreies Wasser, Nicht DEPC-behandelt, Nukleasefreies Wasser, Proteasefreies Wasser, RNasefreies Wasser

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Über diesen Artikel

Lineare Formel:
H2O
CAS-Nummer:
Molekulargewicht:
18.02
NACRES:
NA.31
PubChem Substance ID:
UNSPSC Code:
12191602
EC Number:
231-791-2
MDL number:
Beilstein/REAXYS Number:
2050024
Technique(s):
PCR: suitable, electrophoresis: suitable
Bp:
100 °C (lit.)
Vapor pressure:
3 mmHg
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Technischer Dienst
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Unterstützung erhalten


Produktname

Wasser, Nuclease-Free Water, for Molecular Biology

vapor density

<1 (vs air)

Quality Segment

vapor pressure

3 mmHg

sterility

0.1 μm filtered

form

liquid

technique(s)

PCR: suitable, electrophoresis: suitable

refractive index

n20/D 1.34 (lit.)

pH

5-7

bp

100 °C (lit.)

mp

0 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

foreign activity

DNase, RNase, Protease, free

SMILES string

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

Application

Wasser wird verwendet
  • in der cDNA-Synthese und PCR-Amplifikation
  • für die Verdünnung von Pansen-DNA-Proben vor dem qPCR-Test
  • in der PCR (Polymerase-Kettenreaktion) und Agarose-Gelelektrophorese
  • für die Elution der RNA während der RNA-Isolation

Features and Benefits

  • Nukleasefreies Wasser
  • DNasefrei, RNasefrei, Proteasefrei
  • Nicht DEPC-behandelt
  • Auf 0,1 μm filtriert
  • Hergestellt aus bidestilliertem und entionisiertem Wasser
  • Geeignet für die Verwendung in molekularen Anwendungen

Dieses Produkt ist auf 0,1 μm filtriert. Es ist als Molecular Biology Reagent (molekularbiologisches Reagenz) klassifiziert und eignet sich für Anwendungen in der Molekularbiologie. Dieses Produkt wurde auf die Abwesenheit von Nukleasen und Proteasen geprüft und durchlief eine Keimbelastungsanalyse. Es wurde nicht mit Diethylpyrocarbonat (DEPC) behandelt.

Preparation Note

Dieses Produkt ist aus bidestilliertem und entionisiertem Wasser hergestellt

Other Notes

Die Wasser-Spezifikationstabelle ermöglicht einen einfachen Vergleich der Spezifikationen verschiedener Wasserprodukte.

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Dieser Artikel
9801-OP9528495289
technique(s)

PCR: suitable, electrophoresis: suitable

technique(s)

-

technique(s)

FISH: suitable

technique(s)

-

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

100

form

liquid

form

liquid

form

liquid

form

liquid

pH

5-7

pH

-

pH

5-7

pH

5-7

density

1.000 g/mL at 3.98 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

bp

100 °C (lit.)

bp

100 °C (lit.)

bp

100 °C (lit.)

bp

100 °C (lit.)


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Lagerklasse

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves



Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number

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Questions

1–5 of 5 Questions  
  1. W4502 Millipore Water: what is the ohms of this water?

    1 answer
    1. The conductivity of this water is not determined. Currently, there is no Molecular Biology grade water product available that is tested for conductivity.

      Helpful?

  2. Do you have stability data for W4502 for evaluating if Shelf Life can be extended?

    1 answer
    1. This water product is not assigned an expiration or retest date. In the original sealed container the material is stable nearly indefinitely. Items that are not assigned an expiration date are warranteed for 1 year from the date of shipment.

      Helpful?

  3. Is this water sterile? Can this be used for cell culture?

    1 answer
    1. Product W4502 is not sterile, this product is prepared from Double Distilled and Deionized Water. This product is best suitable to use in molecular biology applications. See product W3500 for Sterile, Tissue Culture grade Water:
      https://www.sigmaaldrich.com/product/sigma/w3500

      Helpful?

  4. In what vessel does this product come? A bottle, a bag with a spigot, etc.?

    1 answer
    1. This product is packaged in square PET square bottles. To request a quote for larger volumes or specialized packaging, please submit a request at the link below:
      https://www.sigmaaldrich.com/services/support/bulk-quotation-request

      Helpful?

  5. This product does not list an expiration date on the label. Could someone provide an expiration for lot number BCCH7145?

    1 answer
    1. This product does not contain an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services

      Helpful?

Reviews

Active Filters

  1. USA
    • Review 1
    • Votes 6
    1 out of 5 stars.

    This water has microbial contamination!

    We used this water (Lot #0000387784) to dilute DNA for cell culture, but the cells kept getting contaminated. Eventually we traced it back to the water. Even a freshly opened bottle had microbial growth after mixing with culture medium. This ended up ruining thousands of dollars of experiments.

    Helpful?

    1. Response from Merck KGaA:

      Thank you for your review. We are sorry to hear that your experience did not match your product expectations. We encourage customers who experience problems with our products to contact us for additional technical support. Please visit https://www.sigmaaldrich.com/US/en/support/customer-support to submit a Product Technical Inquiry.

  2. 1 Ratings-only review