70527

100-mM-IPTG-Lösung – Novagen

Convenient, ready-to-use solution

• Synonym(e): 100-mM-IPTG-Lösung – Novagen, IPTG-Lösung, 100 mM
• CAS-Nummer: 367-93-1
• MDL-Nummer: MFCD00063273
• UNSPSC-Code: 12352200
• NACRES: NA.54

Eigenschaften

• Qualitätsniveau: 100
• Form: solution
• Mol-Gew.: 238.30 g/mol
• Hersteller/Markenname: Novagen^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• SMILES String: S([C@@H]1O[C@@H]([C@@H]([C@@H]([C@H]1O)O)O)CO)C(C)C
• InChI: 1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
• InChIKey: BPHPUYQFMNQIOC-NXRLNHOXSA-N

Beschreibung

Anwendung

100 mM IPTG Solution - Novagen has been used to:

• induce expression of an anti-CD11b-nanobody with a sortase recognition tag in Chalmers (WK6) cells[1]
• induce expression in calcium-independent sortase from BL21(DE3) cells[1]
• turn on bacterial protein production of the protein A/G-MNase[2]

Biochem./physiol. Wirkung

Isopropyl-β-D-1-thiogalactopyranoside (IPTG) is an inducer of the lacoperon. It is highly recommended for blue/white screening using lacZα-complementation in appropriate vectors and host strains, for example, pSTBlue-1/NovaBlue. In addition, it is also used for inducing protein expression with the pET system and various lac promoter-derived expression systems. IPTG is supplied as a sterile aqueous solution and is free of dioxane. It comes in convenient volumes of 10 × 1.5 ml. IPTG is used in bacterial cultures to induce the expression of plasmid-based genes to produce recombinant proteins under the control of the lac promoter.[3]

Warnhinweis

Toxizität: Karzinogen/Teratogen (D)

Rechtliche Hinweise

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable