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S1797

SOC-Medium

For use in transformation

Synonym(e):

Super Optimal broth

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NACRES:
NA.85
UNSPSC Code:
41106200
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grade

Molecular Biology

Quality Segment

500

sterility

0.2 μm filtered

form

liquid

composition

Dextrose, 3.603 g/L , KCl, 0.186 g/L , MgSO4, 4.8 g/L , Tryptone, 20 g/L , Yeast extract, 5 g/L

technique(s)

microbiological culture: suitable

application(s)

agriculture

storage temp.

2-8°C

suitability

nonselective for Escherichia coli, nonselective for coliforms

General description

SOC-Medium (superoptimales Medium mit katabolischem Repressor) ist ein glucosereiches mikrobielles Wachstumsmedium, das primär im Rückgewinnungsschritt bei der Transformation von kompetenten Escherichia-coli-Zellen verwendet wird. Die Kultivierung von E.-coli-Zellen in dieser nährstoffreichen mikrobiellen Bouillon erhöht die Transformationseffizienz rekombinanter Plasmide, die toxische Proteingene enthalten, indem sie die unbeabsichtigte Aktivierung des lac-Promotors auf dem Klonierungsvektor hemmt.[1] SOC-Medium enthält Peptide, Aminosäuren, wasserlösliche Vitamine und Glucose in einer Formulierung mit niedrigem Salzgehalt.

Application

SOC-Medium wird für die Transformation von DH5α-kompetenten E.-coli-Zellen verwendet. [2] Außerdem wird es zur Kultivierung weißer Kolonien nach dem Klonen zur Identifizierung des zu untersuchenden rekombinanten Proteins verwendet.

Features and Benefits

Die SOC-Flüssigkeit bietet Folgendes:
  • Gefiltertes und sterilisiertes gebrauchsfertiges Format
  • Praktische Packungsgrößen
  • Standardmäßige Formulierung

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Dieser Artikel
L254270122L7658
SOC-Medium For use in transformation

Sigma-Aldrich

S1797

SOC-Medium

LB-Bouillon (Miller) Liquid microbial growth medium

Sigma-Aldrich

L2542

LB-Bouillon (Miller)

Nährbouillon NutriSelect® Plus, powder, for bacteria, pack of 100 g, pack of 500 g

Millipore

70122

Nährbouillon

LB-Bouillon EZMix powder microbial growth medium

Sigma-Aldrich

L7658

LB-Bouillon

sterility

0.2 μm filtered

sterility

sterile; 0.2 μm filtered

sterility

non-sterile

sterility

non-sterile

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

Erysipelothrix spp., bacteria, nonselective for Brucella spp., nonselective for Escherichia coli, nonselective for Listeria spp., nonselective for Staphylococcus spp., nonselective for Streptococcus spp., nonselective for bacteria (General Media), nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

application(s)

agriculture

application(s)

microbiology

application(s)

agriculture
bioburden testing
environmental
food and beverages
life science and biopharma
microbiology

application(s)

agriculture
food and beverages
microbiology

form

liquid

form

liquid

form

powder

form

powder

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

storage temp.

2-8°C

storage temp.

room temp

storage temp.

2-25°C

storage temp.

room temp

Lagerklasse

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Questions

1–4 of 4 Questions  

  1. That is the shelf life of SOC medium if kept refrigerated at 4C?

    1 answer

    1. This product is not assigned an expiration date. It is assigned a recommended retest date of approximately 2 years after the quality release date. A recommended retest date is the time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications.

      For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

  2. Why is SOC Medium (S1797) used to recover bacterial cells before transformation?

    1 answer

    1. SOC is a rich bacterial broth that also contains glucose, which aids in the recovery process and prepares the cells for efficient transformation.  The use of other media, such as LB - Lennox may also be acceptable, but the recovery and transformation may not be as efficient.

      Helpful?

  3. Which bacterial culture medium is the best choice for my application?

    1 answer

    1. Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production.  The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

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