跳轉至內容
Merck
全部照片(1)

重要文件

CE0050

Sigma-Aldrich

CelLytic MEM 蛋白提取试剂盒

同義詞:

Membrane protein extraction kit

登入查看組織和合約定價


About This Item

分類程式碼代碼:
41116134
NACRES:
NA.56
暫時無法取得訂價和供貨情況

技術

protein extraction: suitable

品質等級

運輸包裝

wet ice

儲存溫度

−20°C

一般說明

CelLytic MEM蛋白提取试剂盒提供了一种分离细胞中疏水性筏状微结构域相关蛋白的快速、方便的方法。该方法基于相分离,不需要分离细胞膜。分离的蛋白质可用于进一步的实验,例如 SDS-PAGE、蛋白质印迹、斑点印迹和免疫沉淀。 该试剂盒已在 HeLa、HEK-293、NIH 3T3、COS 和 CHO 及其它细胞系上进行了测试。
膜蛋白占据了生物体基因组大约20-30%的成分,充当着胞内的看门人、调节器和感应器的角色。它们具有多样的细胞功能,例如将细胞与外部的毒素隔离开来,作为胞内信号级联的起始点,以及维持临界离子浓度。[1]

應用

CelLytic MEM 蛋白质提取试剂盒已被用于以肝细胞测定脂肪从头 合成[2]和提取用于蛋白质印迹的膜蛋白。[3][4]

適合性

提供足够进行80次检测的试剂。

法律資訊

CelLytic is a trademark of Sigma-Aldrich Co. LLC

僅套裝組件

產品號碼
描述

  • Lysis and Separation Buffer 50 mL

  • Wash Buffer for CelLytic MEM 50 mL

  • Sodium Chloride, 4M Solution 1.5 mL

套裝中的組件也可單獨購買

產品號碼
描述
SDS

  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 1 mLSDS

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2 - Skin Irrit. 2

儲存類別代碼

10 - Combustible liquids

閃點(°F)

188.6 °F - closed cup

閃點(°C)

87 °C - closed cup


從最近期的版本中選擇一個:

分析證明 (COA)

Lot/Batch Number

未看到正確版本?

如果您需要一個特定的版本,您可以透過批號來尋找特定憑證。

已經擁有該產品?

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Willy Morelle et al.
The Journal of clinical endocrinology and metabolism, 102(4), 1375-1386 (2017-03-23)
TMEM165 deficiency is a severe multisystem disease that manifests with metabolic, endocrine, and skeletal involvement. It leads to one type of congenital disorders of glycosylation (CDG), a rapidly growing group of inherited diseases in which the glycosylation process is altered.
Tomoaki Furuta et al.
Cancer science, 112(9), 3722-3731 (2021-06-12)
The rBC2LCN lectin, known as a stem cell marker probe that binds to an H type 3 fucosylated trisaccharide motif, was recently revealed to also bind to pancreatic ductal adenocarcinoma (PDAC) cells. A lectin-drug conjugate was generated by fusing rBC2LCN
Sushmitha Vijaya Kumar et al.
Scientific reports, 10(1), 14985-14985 (2020-09-13)
Membrane organization plays an important role in signaling, transport, and defense. In eukaryotes, the stability, organization, and function of membrane proteins are influenced by certain lipids and sterols, such as cholesterol. Bacteria lack cholesterol, but carotenoids and hopanoids are predicted
Hiroaki Tateno et al.
Glycobiology, 26(12), 1328-1337 (2016-04-03)
Human somatic stem cells such as human mesenchymal stem cells (hMSCs) are considered attractive cell sources for stem cell-based therapy. However, quality control issues have been raised concerning their safety and efficacy. Here we used lectin microarray technology to identify
Elisabeth P Carpenter et al.
Current opinion in structural biology, 18(5), 581-586 (2008-08-05)
Membrane protein structural biology is still a largely unconquered area, given that approximately 25% of all proteins are membrane proteins and yet less than 150 unique structures are available. Membrane proteins have proven to be difficult to study owing to

Questions

Reviews

No rating value

Active Filters

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務