As mentioned in the 'DESCRIPTION' section, the composition of this product is considered proprietary and may not be shared. This product does not contain any denaturants except for a proprietary mild detergent. Lysates obtained with this product may be immediately run on a SDS PAGE after the addition of loading buffer.
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一般說明
CelLytic M是一种专有的去污剂溶液，专为从培养的哺乳动物细胞中提取有效的全细胞蛋白而设计。 它能够对悬浮细胞和贴壁细胞进行有效和快速的细胞裂解和蛋白质溶解。 裂解物可用于许多下游应用而无需对CelLytic M进行去除，例如报告基因测定、Western印迹/免疫沉淀、凝胶阻滞实验、磷酸酶测定、以及激酶测定。
特點和優勢
- 高效：效率比冻融、超声和其他产品最多高50％
- 非变性：不会干扰下游应用，如免疫沉淀、激酶和磷酸酶测定、报告基因检测、以及凝胶阻滞实验
- 方便：即用型试剂无需从培养板上刮下
- 快速：可在室温下快速裂解细胞。
數量
用于蛋白质提取的CelLytic M试剂的效率已经在但不限于HeLa、CHO、COS、HL-60、Jurkat、A431、PC-12和牛主动脉内皮细胞（BAEC）上进行了测试。
分析報告
106-107 悬浮细胞使用125μl CelLytic M 。 对于贴壁细胞，在100 mm平板中使用500-1,000μL；在35 mm平板中适用200-400μL。
法律資訊
CelLytic is a trademark of Sigma-Aldrich Co. LLC
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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分析證明 (COA)
Lot/Batch Number
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ReadyShield® 磷酸酶和蛋白酶抑制劑雞尾酒常見問題，用於各種細胞類型和組織萃取物的樣本保護，包括哺乳動物、植物和微生物樣本。我們的 ReadyShield® Protease Inhibitor Cocktail 是一種非冷凍溶液，含有對絲氨酸、半胱氨酸、酸性蛋白酶和氨肽酶具有廣泛特異性的抑制劑。
ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.
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Does the CelLytic M (Cat # 2978) contain DTT, and if it does, at what concentration?
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Is the product compatible with the BCA assay? Additionally, are there any denaturants in the formulation?
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CelLytic™ M is suitable for Bradford Assay and BCA assays. For the Lowry method, there is a background issue that is problematic for low protein concentrations, but may be subtracted with high protein concentrations.
CelLytic™ M does not contain any primary amines; it is a buffered solution (pH 7.6) which has no denaturants except for a mild detergent. The exact formulation is proprietary. This product may be diluted or dialyzed if it is found to have interference in your particular assay.Helpful?
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Is the C2978 lysis buffer suitable for viral DNA extraction from cultured mammalian cells (fewer than 25,000 cells/ul) and subsequent qPCR?
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The cellular DNA is centrifuged down and will not be present in the supernatant along with the proteins. To isolate the DNA, a different kit is necessary.
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Does the CelLytic M (Cat. No.: 2978) contain SDS, and if it does, at what concentration?
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The product with the code C2978, CelLytic M, does not contain SDS. Instead, it contains a mild detergent in a buffer.
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What is the critical micelle concentration (CMC) of the product?
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Unfortunately, the CMC value has not been tested for this product.
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Has CelLytic been tested for neutralizing HIV or use in a BSL3 facility?
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We have not tested CelLytic for neutralizing HIV nor used in a BSL3 facility.
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Is there any denaturants in Product C2978, CelLytic™ M that can interfere with my downstream applications?
1 answer-
CelLytic™ M does not contain any primary amines; it is a buffered solution (pH 7.6) which has no denaturants except for a mild detergent. The exact formulation is proprietary. This product may be diluted or dialyzed if it is found to have interference in your particular assay.
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Will CelLytic™ M, Product No. C2978, interfere with protein concentration assays such as Bradford, Lowry and BCA?
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We have tested and found CelLytic™ M to be compatible for Bradford Assay and BCA assays. For the Lowry method, there is a background issue that is problematic for low protein concentrations, but may be subtracted with high protein concentrations.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Will Product No. C2978, CelLytic™ M solubilize membrane proteins?
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CelLytic™ M can solubilize membrane proteins as well as cytoplasmic proteins and the conditions are non-denaturing. However, we cannot guarantee the solubilization of all membrane proteins. The following proteins were detected in the CelLytic™ M lysate when tested with these antibodies against membrane proteins: Placental alkaline phosphatase, CD45, Connexin and Caveolin.
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