推薦產品
技術
protein extraction: suitable
儲存溫度
−20°C
一般說明
植物总蛋白抽提试剂盒（Plant Total Protein Extraction Kit）是专为植物生命科学应用而设计的产品，能够从任意植物种类或组织中抽提蛋白定性样本。本方案无需任何超速离心步骤或含水多聚物的两相分离步骤（APTP）。本试剂盒包含两类试剂：一份植物特异性的蛋白酶抑制剂cocktail、一份增加了溶解能力以抽提更多疏水蛋白质的全新离液剂（C0356, 蛋白抽提试剂第4类试剂，Protein Extraction Reagent Type 4). 在植物组织样品顺序蛋白抽提过程中采用新鲜试剂，能洗脱植物组织中的几乎所有蛋白质。蛋白酶抑制剂cocktail是作用于丝氨酸、半胱氨酸、金属蛋白酶、天冬氨酸与氨肽酶等酶类的多种蛋白酶的广谱抑制剂混合物。本品含有胃酶抑素A，4-（2-氨基乙基）苯磺酰氟（AEBSF）中，E-64，亮肽素，1,10-菲咯啉，和苯丁抑制素等多种成分。经验证，cocktail抑制剂混合物能够在抽提过程中高效避免蛋白降解的发生。
在去除了多酚，鞣质等干扰物质之后，新鲜或冻存的植物组织经研磨后，用离液剂重悬。通过离心沉淀植物碎片，并收集蛋白抽提液。最后用户可获得定性的分级蛋白样品，用于下游蛋白组学分析。
在去除了多酚，鞣质等干扰物质之后，新鲜或冻存的植物组织经研磨后，用离液剂重悬。通过离心沉淀植物碎片，并收集蛋白抽提液。最后用户可获得定性的分级蛋白样品，用于下游蛋白组学分析。
應用
植物总蛋白提取试剂盒用于分离种子粗蛋白。还用于提取总蛋白。
數量
足够用于从10-250mg的植物组织样品中制备20个单独的提取物
訊號詞
Warning
危險分類
Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Repr. 2
儲存類別代碼
11 - Combustible Solids
閃點（°F）
185.0 °F - closed cup
閃點（°C）
85 °C - closed cup
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
客戶也查看了
Revealing proteins associated with symbiotic germination of Gastrodia elata by proteomic analysis
Zeng X, et al.
Botanical studies, 59(1), 8-8 (2018)
Samuel L Chen et al.
Frontiers in plant science, 10, 1116-1116 (2019-10-15)
Pre-mRNA alternative splicing is a conserved mechanism for eukaryotic cells to leverage existing genetic resources to create a diverse pool of protein products. It is regulated in coordination with other events in RNA metabolism such as transcription, polyadenylation, RNA transport
Xu Zeng et al.
Botanical studies, 59(1), 8-8 (2018-03-08)
Gastrodia elata, a mycoheterotrophic orchid, is a well-known medicinal herb. In nature, the seed germination of G. elata requires proper fungal association, because of the absence of endosperm. To germinate successfully, G. elata obtains nutrition from mycorrhizal fungi such as
Peitong Wang et al.
Plant physiology, 178(4), 1568-1583 (2018-10-13)
Arsenic (As) is highly toxic to plants and detoxified primarily through complexation with phytochelatins (PCs) and other thiol compounds. To understand the mechanisms of As toxicity and detoxification beyond PCs, we isolated an arsenate-sensitive mutant of Arabidopsis (Arabidopsis thaliana), arsenate
Rishiram Ramanan et al.
Plant physiology, 177(3), 1050-1065 (2018-05-18)
Algae undergo a complete metabolic transformation under stress by arresting cell growth, inducing autophagy and hyper-accumulating biofuel precursors such as triacylglycerols and starch. However, the regulatory mechanisms behind this stress-induced transformation are still unclear. Here, we use biochemical, mutational, and
我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
聯絡技術服務