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重要文件

MCL1

Sigma-Aldrich

哺乳动物细胞裂解试剂盒

同義詞:

Cell Lysis Reagent

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.56
暫時無法取得訂價和供貨情況

用途

 kit sufficient for 250 extractions (100 mm tissue culture plates)

品質等級

技術

immunoprecipitation (IP): suitable

運輸包裝

dry ice

儲存溫度

−20°C

應用

本产品含有分解哺乳动物细胞和组织以便于之后分析蛋白目标基因所需的一切试剂。在裂解过程中,胞内蛋白保持稳定,适于进行免疫检测、免疫沉淀、亲和纯化及其他功能检测。
哺乳动物细胞裂解试剂盒以用于检测血管内皮生长因子(VEGF) 受体-1及其配体VEGF-B在活动神经元退化过程中发挥的作用。[1]

特點和優勢

  • 该试剂盒可用于制备细胞裂解缓冲液和其他改性缓冲液。
  • 该试剂盒允许研究人员调整缓冲液成分,从而获得对于特定蛋白质最高效的缓冲液。

成分

哺乳动物细胞理解试剂盒包含各种可以混合的溶液,可用于裂解贴壁、未贴壁的细胞及组织。其含有缓冲液、去垢剂、Nacl、蛋白质抑制物混合溶液。

套裝中的組件也可單獨購買

產品號碼
描述
SDS

  • P8340Protease inhibitor cocktail 2.5 mLSDS

象形圖

CorrosionExclamation mark

訊號詞

Danger

危險分類

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

10 - Combustible liquids

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Shintaro Nakao et al.
The American journal of pathology, 178(4), 1913-1921 (2011-03-26)
Vascular adhesion protein-1 (VAP-1) contributes to inflammatory and angiogenic diseases, including cancer and age-related macular degeneration. It is expressed in blood vessels and contributes to inflammatory leukocyte recruitment. The cytokines IL-1β and vascular endothelial growth factor A (VEGF-A) modulate angiogenesis
Lech Martyniszyn et al.
Viral immunology, 26(5), 322-335 (2013-10-15)
Several studies have provided evidence that complex relationships between autophagic and apoptotic cell death pathways occur in cancer and virus-infected cells. Previously, we demonstrated that infection of macrophages with Moscow strain of ectromelia virus (ECTV-MOS) induces apoptosis under in vitro
Shintaro Nakao et al.
Blood, 117(3), 1081-1090 (2010-08-14)
Angio- and lymphangiogenesis are inherently related processes. However, how blood and lymphatic vessels regulate each other is unknown. This work introduces a novel mechanism explaining the temporal and spatial relation of blood and lymphatic vessels. Vascular endothelial growth factor-A (VEGF-A)
Shintaro Nakao et al.
Investigative ophthalmology & visual science, 54(6), 3830-3836 (2013-04-13)
Inflammation and immune cells regulate choroidal neovascularization (CNV) and could become therapeutic targets in age-related macular degeneration (AMD). Lymphangiogenesis is a key component of various inflammatory diseases. Whether lymphangiogenesis and lymph node-mediated immunity are involved in the pathogenesis of AMD
Shinsuke Yasuda et al.
Journal of immunology (Baltimore, Md. : 1950), 179(7), 4890-4900 (2007-09-20)
Dysregulation of Ras guanyl nucleotide-releasing protein 1 (RasGRP1) in mice results in a systemic lupus erythematosus (SLE)-like disorder. We therefore looked for defective isoforms and/or diminished levels of human RasGRP1 in a cohort of SLE patients. PBMCs were collected from

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