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70584-M

Millipore

BugBuster®蛋白提取试剂

同義詞:

Protein Extraction Reagent, Protein Extraction Solution

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About This Item

分類程式碼代碼:
41106516
NACRES:
NA.32

形狀

liquid

品質等級

100

製造商／商標名

Novagen®

儲存條件

OK to freeze
avoid repeated freeze/thaw cycles

儲存溫度

10-30°C

相關類別

一般說明

BugBuster®蛋白提取试剂是为温和地破坏大肠杆菌细胞壁并释放活性蛋白而配制的。它提供了一种简单、快速、低成本的替代机械方法，如弗氏压碎器或超声波法，用于释放表达的目标蛋白，以制备纯化或其他应用。该专利配方采用非离子和两性离子洗涤剂的三缓冲基混合物，能够在蛋白不变性的情况下完成细胞壁穿孔。BugBuster还可用于制备高纯度包涵体，用于表达的蛋白质不溶的情况下进行后续纯化。

應用

BugBuster®蛋白提取试剂已用于：
  • 裂解大肠杆菌BLR (DE3)细胞制备β-葡萄糖苷酶(BglB)并纯化
  • 作为细胞裂解缓冲液，处理聚囊藻PCC6803转偶联物，并测定黄色荧光蛋白(YFP)的荧光
  • 谷胱甘肽-转移酶-热休克蛋白家族A (Hsp70)成员8 (GST- HSPA8)的裂解缓冲液

警告

毒性：标准处理（A）

法律資訊

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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Patricia Armshaw et al.
Biotechnology for biofuels, 8, 201-201 (2015-12-03)
The use of photosynthetic autotrophs and in particular the model organism Synechocystis PCC6803 is receiving much attention for the production of sustainable biofuels and other economically useful products through metabolic engineering. Optimisation of metabolic-engineered organisms for high-level sustained production of
Dylan Alexander Carlin et al.
PloS one, 12(5), e0176255-e0176255 (2017-05-23)
Accurate modeling of enzyme activity and stability is an important goal of the protein engineering community. However, studies seeking to evaluate current progress are limited by small data sets of quantitative kinetic constants and thermal stability measurements. Here, we report
Tiejian Nie et al.
Autophagy, 17(10), 2923-2938 (2021-05-11)
As a highly dynamic organelle, mitochondria undergo constant fission and fusion to change their morphology and function, coping with various stress conditions. Loss of the balance between fission and fusion leads to impaired mitochondria function, which plays a critical role

文章

引文聚焦：BugBuster® 蛋白提取試劑可從細菌病原體中高效提取蛋白質

引文聚焦：BugBuster® 蛋白提取試劑可從細菌病原體中高效提取蛋白質

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

BugBuster® and Benzonase® Reagents are the Clear Solutions to Simple, Efficient Extraction of E. coli Proteins

This article shows the use of BugBuster® and Benzonase® as protein purification tools to extract recombinant proteins from E. coli and to reduce the viscosity of the extract.

ReadyShield 磷酸酶和蛋白酶抑制劑混合液常見問題集

ReadyShield® 磷酸酶和蛋白酶抑制劑雞尾酒常見問題，用於各種細胞類型和組織萃取物的樣本保護，包括哺乳動物、植物和微生物樣本。我們的 ReadyShield® Protease Inhibitor Cocktail 是一種非冷凍溶液，含有對絲氨酸、半胱氨酸、酸性蛋白酶和氨肽酶具有廣泛特異性的抑制劑。

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相關內容

裂解與蛋白質萃取

細胞裂解與蛋白質萃取方法概述各種技術，從洗滌劑溶解到機械破壞，支援研究需求。

Lysis & Protein Extraction

Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.

我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.

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