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Merck
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重要文件

11284932001

Roche

DNase I

from bovine pancreas

同義詞:

脱氧核糖核酸酶

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About This Item

酶委員會編號:
分類程式碼代碼:
12352204
暫時無法取得訂價和供貨情況

生物源

bovine pancreas

品質等級

形狀

lyophilized

包裝

pkg of 100 mg

製造商/商標名

Roche

技術

cell culture | mammalian: suitable

儲存溫度

2-8°C

相關類別

一般說明

DNase I是一种可降解DNA的双链特异性内切核酸酶。牛胰腺脱氧核糖核酸酶I(DNase I)是DNA小沟相互作用的核酸酶,特异性相对较低。该蛋白质由两个中心β折叠构成,而每个折叠由六个β-链所组成。该结构被大量的环以及α-螺旋区域包围。该酶与核酸外切酶III具有结构相似性。[1]DNase I是研究得最透彻的哺乳动物来源的内切酶之一。[2]

應用

DNase I 与胶原酶一起已被用于组织解离。[3][4][5]

生化/生理作用

在组织解离过程中,部分细胞被裂解并导致DNA的释放。单分子DNA可能会导致细胞结团。向解离缓冲液中加入DNase I会导致该细胞外DNA的降解,因而避免了因意外结团造成的细胞损失。[6][7][8]

特點和優勢

  • 冻干的
  • 且冻干前经 0.2 μm 孔径膜过滤
  • 糖蛋白和双链特异性内切酶

單位定義

一个单位表示在测定条件下导致吸光度以0.001每分钟的速率而增加的酶活力。

準備報告

激活剂:二价金属离子 (Ca2+, Mg2+)
工作浓度:0.01 至 1 mg/ml
注意:必须针对每种细胞类型单独确定工作浓度。为实现最佳活性,酶须添加 5 mM Mg2+

重構

用无菌的双蒸水对冻干酶进行重悬(10 mg/ml);用PBS(磷酸盐缓冲液)、HBSS(Hank′s 平衡盐溶液)或培养基进行进一步的稀释。

其他說明

仅用于生命科学研究。不用于诊断操作。

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Resp. Sens. 1 - Skin Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

does not flash

閃點(°C)

does not flash


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