T9777

Trypsin-chymotrypsin inhibitor

lyophilized powder

• Synonim(y): Bowman-Birk Inhibitor
• Numer CAS: 37330-34-0
• Numer MDL: MFCD00132532
• Kod UNSPSC: 12352202
• NACRES: NA.77

Właściwości

• Nazwa produktu: Trypsin-chymotrypsin inhibitor from Glycine max (soybean), lyophilized powder
• pochodzenie biologiczne: Glycine max (soybean)
• Poziom jakości: 200
• Formularz: lyophilized powder
• skład: Protein, 70-90% biuret
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

Highly cross-linked with seven disulfide bridges, Bowman-Birk inhibitor has spatially distinct domains for trypsin and chymotrypsin inhibition.

Trypsin-chymotrypsin inhibitor belongs to the family of plant protease inhibitors. It is mainly found in the legume (Fabaceae) and cereal (Poaceae) families. Trypsin-chymotrypsin inhibitor consists of many internal disulfide bonds between conserved Cys residues, which maintains structural stability and active conformation of the inhibitory loops.[1] Trypsin-chymotrypsin has anticarcinogenic properties.[2]

Zastosowanie

Bowman Birk protease inhibitor prevents radiation-induced carcinogenesis by a reduction of incorrect DNA repairs, resulting in a reduced amount of dicentric chromosomes.

Trypsin-chymotrypsin inhibitor from Glycine max (soybean) has been used for flow cytometric separase activity assay.[3] It has also been used to inhibit trypsin and chymotrypsin for in vitro gastrointestinal (GI) digestion studies.[4]

Definicja jednostki

One trypsin unit = ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C. Reaction volume = 3.2 mL (1 cm light path).

Postać fizyczna

Lyophilized powder containing phosphate buffer salts, pH 7.6

Komentarz do analizy

1 mg protein will inhibit ≥0.5 mg trypsin with activity of ~10,000 BAEE units per mg protein or ≥1.0 mg chymotrypsin with activity of ~40 BTEE units per mg protein.

Inne uwagi

View more information on Trypsin Inhibitor.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)